{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:25:54Z","timestamp":1761060354423,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T00:00:00Z","timestamp":1499126400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["146632"],"award-info":[{"award-number":["146632"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Doublecortin (<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content>) is a microtubule\u2010associated protein widely used as an indicator of neurogenesis in immunohistochemical analyses of the postmortem adult brain. A recent study reported that <jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> can be quantified in the cerebrospinal fluid (<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>) from healthy rats between postnatal day 0 (P0) and P30. However, it is currently unclear whether the concentration of <jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content>) may represent a measure of endogenous neurogenesis. To address this question, this study examined the impact of a neonatal hypoxic\u2010ischemic (<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>) brain injury, known to induce neurogenesis, on <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content> was elicited at P7 in Sprague\u2013Dawley rat neonates, and <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> was collected serially from the cisterna magna at P5 and P10, or at P10 and P15. A sandwich immunoassay was used to measure <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content>. Brains from P10 neonates were analyzed immunohistochemically for neurogenesis and cell death markers. Mean <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> was significantly higher in <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>\u2010 than in sham\u2010exposed animals, at both P10 and P15. In the <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content> group at P10, <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> and stroke severity correlated positively. <jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> immunoreactivity was increased in the ipsilateral neurogenic niches from the P10 <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content> brains in comparison with that of shams. The number of proliferative <jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content>\u2010positive cells was higher in the ipsilateral hippocampal subgranular zone (<jats:styled-content style=\"fixed-case\">SGZ<\/jats:styled-content>) than in the <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content> contralateral or sham <jats:styled-content style=\"fixed-case\">SGZ<\/jats:styled-content>. Thus, neonatal <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content> brain injury disrupts the developmental time\u2010course of <jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> levels in the <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>. Our data suggest that the increased concentration of <jats:styled-content style=\"fixed-case\">DCX<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> after neonatal <jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content> is the result of both cellular injury and increased neurogenesis.<\/jats:p>","DOI":"10.1111\/ejn.13612","type":"journal-article","created":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T11:12:46Z","timestamp":1495797166000},"page":"1758-1767","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Neonatal hypoxia\u2010ischemia in rat increases doublecortin concentration in the cerebrospinal fluid"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0030-666X","authenticated-orcid":false,"given":"Catherine","family":"Br\u00e9g\u00e8re","sequence":"first","affiliation":[{"name":"Brain Ischemia and Regeneration Department of Biomedicine and Department of Neurosurgery University Hospital Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urs","family":"Fisch","sequence":"additional","affiliation":[{"name":"Brain Ischemia and Regeneration Department of Biomedicine and Department of Neurosurgery University Hospital Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin H.","family":"Sailer","sequence":"additional","affiliation":[{"name":"Schulthess Clinic Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang S.","family":"Lieb","sequence":"additional","affiliation":[{"name":"Brain Ischemia and Regeneration Department of Biomedicine and Department of Neurosurgery University Hospital Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie","family":"Chicha","sequence":"additional","affiliation":[{"name":"Brain Ischemia and Regeneration Department of Biomedicine and Department of Neurosurgery University Hospital Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabienne","family":"Goepfert","sequence":"additional","affiliation":[{"name":"Roche Pharmaceutical Research and Early Development Roche Innovation Center Basel F. 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