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Vomeronasal\u2013accessory olfactory bulb inputs to the medial amygdala (Me) regulate courtship in female rodents. We used a reversible inhibitory chemogenetic technique (Designer Receptors Exclusively Activated by Designer Drugs; <jats:styled-content style=\"fixed-case\">DREADD<\/jats:styled-content>s) to assess the contribution of Me signaling to females\u2019 preference for male pheromones and improvement in receptivity normally seen with repeated testing. Sexually na\u00efve females received bilateral Me injections of an adeno\u2010associated virus carrying an inhibitory <jats:styled-content style=\"fixed-case\">DREADD<\/jats:styled-content>. Females were later ovariectomized, treated with ovarian hormones, and given behavioral tests following intraperitoneal injections of saline or clozapine\u2010N\u2010oxide (<jats:styled-content style=\"fixed-case\">CNO<\/jats:styled-content>; which hyperpolarizes infected Me neurons). <jats:styled-content style=\"fixed-case\">CNO<\/jats:styled-content> attenuated females\u2019 preference to investigate male vs. female urinary odors. Repeated <jats:styled-content style=\"fixed-case\">CNO<\/jats:styled-content> treatment also slowed the increase in lordosis otherwise seen in females given saline. However, when saline was given to females previously treated with <jats:styled-content style=\"fixed-case\">CNO<\/jats:styled-content>, their lordosis quotients were as high as other females repeatedly given saline. No disruptive behavioral effects of <jats:styled-content style=\"fixed-case\">CNO<\/jats:styled-content> were seen in estrous females lacking <jats:styled-content style=\"fixed-case\">DREADD<\/jats:styled-content> infections of the Me. Finally, <jats:styled-content style=\"fixed-case\">CNO<\/jats:styled-content> attenuated the ability of male pheromones to stimulate Fos expression in the Me of <jats:styled-content style=\"fixed-case\">DREADD<\/jats:styled-content>\u2010infected mice but not in non\u2010infected females. Our results affirm the importance of Me signaling in females\u2019 chemosensory preferences and in the acute expression of lordosis. However, they provide no indication that Me signaling is required for the increase in receptivity normally seen after repeated hormone priming and testing with a male.<\/jats:p>","DOI":"10.1111\/ejn.13636","type":"journal-article","created":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T08:22:42Z","timestamp":1499242962000},"page":"2035-2046","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["<scp>DREADD<\/scp>\u2010induced silencing of the medial amygdala reduces the preference for male pheromones and the expression of lordosis in estrous female mice"],"prefix":"10.1111","volume":"46","author":[{"given":"Elizabeth A.","family":"McCarthy","sequence":"first","affiliation":[{"name":"Department of Biology Boston University Boston MA 02215 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arman","family":"Maqsudlu","sequence":"additional","affiliation":[{"name":"Department of Biology Boston 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