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The initial drug experience is suggested to predict vulnerability of developing addiction, but only few studies have assessed the perception of reward accompanying this initial exposure. We recently presented an approach to assess the initial rewarding effects of cocaine in mice with a single\u2010exposure place preference (se<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>) protocol, avoiding repeated drug injections. Here, we demonstrate a condensed version of the se<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>, allowing assessment of initial subjective reward\u2010perception within a day. By using this protocol, we demonstrate that a single exposure to both cocaine and amphetamine is sufficient to induce place preference. Furthermore, we use chemogenetics ( Designer Receptors Exclusively Activated by Designer Drugs [<jats:styled-content style=\"fixed-case\">DREADD<\/jats:styled-content>]) to show that both inhibitory and stimulatory modulation of<jats:styled-content style=\"fixed-case\">VTA DA<\/jats:styled-content>signalling disrupts cocaine\u2010induced place preference in the condensed se<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>. Our findings support the presence of initial reward\u2010perception of both cocaine and amphetamine, and the formation of drug\u2010context association. In addition, our data support that<jats:styled-content style=\"fixed-case\">VTA DA<\/jats:styled-content>signalling prior to drug exposure affects either reward\u2010perception or the time during which associations are formed, thereby preventing induction of cocaine\u2010induced place preference in the se<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>. The easy and timesaving se<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>protocol should form a critical basis for further deciphering the complex mechanisms underlying the progression from the initial drug experience to escalating drug intake and addiction.<\/jats:p>","DOI":"10.1111\/ejn.14082","type":"journal-article","created":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T10:06:25Z","timestamp":1532513185000},"page":"2156-2163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Initial rewarding effects of cocaine and amphetamine assessed in a day using the single\u2010exposure place preference protocol"],"prefix":"10.1111","volume":"50","author":[{"given":"Annika H.","family":"Runegaard","sequence":"first","affiliation":[{"name":"Faculty of Health and Medical Sciences Molecular Neuropharmacology and Genetics Laboratory Department of Neuroscience Panum Institute \u2013 Maersk Tower 7.5 University of Copenhagen Blegdamsvej 3 Copenhagen DK\u20102200 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrine Louise","family":"Jensen","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences Molecular Neuropharmacology and Genetics Laboratory Department of Neuroscience Panum Institute \u2013 Maersk Tower 7.5 University of Copenhagen Blegdamsvej 3 Copenhagen DK\u20102200 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gitta","family":"W\u00f6rtwein","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropsychiatry Psychiatric Center Copenhagen and Department of Neuroscience University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrik","family":"Gether","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences Molecular Neuropharmacology and Genetics Laboratory Department of Neuroscience Panum Institute \u2013 Maersk Tower 7.5 University of Copenhagen Blegdamsvej 3 Copenhagen DK\u20102200 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2006.12.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700293104"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00310636"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002130050917"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002130100720"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015870"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90012-2"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600905103"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/dar.12088"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.5274"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2004.06.032"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-07-02873.2002"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2014.07.016"},{"key":"e_1_2_10_15_1","first-page":"11","article-title":"Cocaine locomotor activation, sensitization and place preference in six inbred strains of mice","volume":"7","author":"Eisner\u2010Dorman A.F.","year":"2011","journal-title":"Behav. 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