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Previous studies conducted in other cities indicated college students as a group at high risk for substance (mis\u2010)use that was associated with individual psychological and cognitive impairments as well as lower academic performance. The aim of this study was to provide detailed data about the substance use patterns of Berlin college students. In addition, major protective and risk factors were analysed. An online questionnaire assessing sociodemographic data and various relevant aspects of both legal and illegal substance use such as consumption pattern and frequency as well as risk\u2010taking behaviour was developed and distributed among colleges in Berlin. A sample of 9351 participants from 17 different colleges in Berlin completed the questionnaire. The study revealed high lifetime (69.3%), past year (45.9%) and past month (28.3%) prevalence of illicit substance use in the sample. Daily tobacco\u2010smoking, a mental disorder diagnosis, a positive screening for problematic consumption (Cage\u2010<jats:styled-content style=\"fixed-case\">AID<\/jats:styled-content>), bisexual orientation and living in open relationship were main factors positively associated with the prevalence and the extent of illicit substance use. Students in Berlin appear to show higher rates of illicit substance use than was previously reported for age\u2010matched individuals in the general German population and college students in other cities. 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