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To test a possible role of synaptic vesicle in <jats:styled-content style=\"fixed-case\">CICR<\/jats:styled-content>, we examined the effects of loading of <jats:styled-content style=\"fixed-case\">EGTA<\/jats:styled-content>, a Ca<jats:sup>2+<\/jats:sup> chelator, into synaptic vesicles and depolymerization of actin fibers. Intravesicular <jats:styled-content style=\"fixed-case\">EGTA<\/jats:styled-content> loading via endocytosis inhibited the ryanodine sensitive enhancement of transmitter release induced by tetanic stimulation and the associated rises in intracellular\u2010free Ca<jats:sup>2+<\/jats:sup> ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>: Ca<jats:sup>2+<\/jats:sup> transients). Latrunculin A, a depolymerizer of actin fibers, enhanced both spontaneous and stimulation\u2010induced transmitter release, but inhibited the enhancement of transmitter release elicited by successive tetanic stimulation. The results suggest a possibility that the activation of <jats:styled-content style=\"fixed-case\">CICR<\/jats:styled-content> from mobilized synaptic vesicles caused the enhancement of neurotransmitter release.<\/jats:p>","DOI":"10.1111\/ejn.14353","type":"journal-article","created":{"date-parts":[[2019,1,28]],"date-time":"2019-01-28T07:49:29Z","timestamp":1548661769000},"page":"1700-1711","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of intravesicular loading of a Ca<sup>2+<\/sup> chelator and depolymerization of actin fibers on neurotransmitter release in frog motor nerve terminals"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0354-1000","authenticated-orcid":false,"given":"Kazuhiko","family":"Narita","sequence":"first","affiliation":[{"name":"Department of Physiology Kawasaki Medical School Kurashiki 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