{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T22:54:29Z","timestamp":1786834469933,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T00:00:00Z","timestamp":1562198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571193"],"award-info":[{"award-number":["81571193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2019,11]]},"abstract":"Abstract<\/jats:title>\n \n Olfactory ensheathing cells (\n OEC<\/jats:styled-content>\n s) are a specialized class of glia, wrapping around olfactory sensory axons that target the olfactory bulb (\n OB<\/jats:styled-content>\n ) and cross the peripheral nervous system\/central nervous system boundary during development and continue to do so post\u2010natally.\n OEC<\/jats:styled-content>\n subpopulations perform distinct subtype\u2010specific functions dependent on their maturity status. Disrupted\n OEC<\/jats:styled-content>\n development is thought to be associated with abnormal\n OB<\/jats:styled-content>\n morphogenesis, leading to anosmia, a defining characteristic of Kallmann syndrome. Hence, anosmin\u20101 encoded by Kallmann syndrome gene (\n \n KAL<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n ) might modulate\n OEC<\/jats:styled-content>\n differentiation\/maturation in the\n OB<\/jats:styled-content>\n . We performed\n in ovo<\/jats:italic>\n electroporation of sh\n RNA<\/jats:styled-content>\n in the olfactory placode to knock\u2010down\n kal<\/jats:italic>\n in chick embryos, resulting in abnormal\n OB<\/jats:styled-content>\n morphogenesis and loss of olfactory sensory axonal innervation into\n OB<\/jats:styled-content>\n .\n BLBP<\/jats:styled-content>\n \u2010expressing\n OEC<\/jats:styled-content>\n s appeared to form a thinner and poorly organized outmost\n OB<\/jats:styled-content>\n layer where\n SOX<\/jats:styled-content>\n 10 expressing\n OEC<\/jats:styled-content>\n s were completely absent with emergence of\n GFAP<\/jats:styled-content>\n \u2010expressing\n OEC<\/jats:styled-content>\n s. Furthermore, in embryonic day 10 chick\n OB<\/jats:styled-content>\n explant cultures,\n GFAP<\/jats:styled-content>\n expression in\n OEC<\/jats:styled-content>\n s accumulating along the\n OB<\/jats:styled-content>\n nerve layers was dramatically reduced by recombinant anosmin\u20101. We then purified immature\n OEC<\/jats:styled-content>\n s from embryonic day 10 chick\n OB<\/jats:styled-content>\n . These cells express\n GFAP<\/jats:styled-content>\n after 7\u00a0days in vitro, exhibiting a multipolar morphology. Overexpression of chick anosmin, exogenous anosmin\u20101 or\n FGF<\/jats:styled-content>\n 2 could inhibit\n GFAP<\/jats:styled-content>\n expression with cells presenting elongated morphology, which was blocked by the\n FGF<\/jats:styled-content>\n receptor inhibitor Su5402. These data demonstrate that anosmin\u20101 functions via\n FGF<\/jats:styled-content>\n signalling in regulating\n OEC<\/jats:styled-content>\n maturation, thereby providing a permissive glial environment for axonal innervation into the\n OB<\/jats:styled-content>\n during development.\n <\/jats:p>","DOI":"10.1111\/ejn.14483","type":"journal-article","created":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T06:13:51Z","timestamp":1560492831000},"page":"3472-3486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Extracellular matrix protein anosmin\u20101 modulates olfactory ensheathing cell maturation in chick olfactory bulb development"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5507-6123","authenticated-orcid":false,"given":"Youli","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Affiliated Hospital of Nanjing Medical 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