{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:04:10Z","timestamp":1747184650002,"version":"3.40.5"},"reference-count":95,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,11,3]],"date-time":"2019-11-03T00:00:00Z","timestamp":1572739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> channels (VGCCs) are considered to play a key role in auditory perception and information processing within the murine inner ear and brainstem. In the past, Ca<jats:sub>v<\/jats:sub>1.3 L\u2010type VGCCs gathered most attention as their ablation causes congenital deafness. However, isolated patch\u2010clamp investigation and localization studies repetitively suggested that Ca<jats:sub>v<\/jats:sub>2.3 R\u2010type VGCCs are also expressed in the cochlea and further components of the ascending auditory tract, pointing to a potential functional role of Ca<jats:sub>v<\/jats:sub>2.3 in hearing physiology. Thus, we performed auditory profiling of Ca<jats:sub>v<\/jats:sub>2.3<jats:sup>+\/+<\/jats:sup> controls, heterozygous Ca<jats:sub>v<\/jats:sub>2.3<jats:sup>+\/\u2212<\/jats:sup> mice and Ca<jats:sub>v<\/jats:sub>2.3 null mutants (Ca<jats:sub>v<\/jats:sub>2.3<jats:sup>\u2212\/\u2212<\/jats:sup>) using brainstem\u2010evoked response audiometry. Interestingly, click\u2010evoked auditory brainstem responses (ABRs) revealed increased hearing thresholds in Ca<jats:sub>v<\/jats:sub>2.3<jats:sup>+\/\u2212<\/jats:sup> mice from both genders, whereas no alterations were observed in Ca<jats:sub>v<\/jats:sub>2.3<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Similar observations were made for tone burst\u2010related ABRs in both genders. However, Ca<jats:sub>v<\/jats:sub>2.3 ablation seemed to prevent mutant mice from total hearing loss particularly in the higher frequency range (36\u201342\u00a0kHz). Amplitude\u00a0growth function analysis revealed, i.a., significant reduction in ABR wave W<jats:sub>I<\/jats:sub> and W<jats:sub>III<\/jats:sub> amplitude in mutant animals. In addition, alterations in W<jats:sub>I<\/jats:sub>\u2010W<jats:sub>IV<\/jats:sub> interwave interval were observed in female Ca<jats:sub>v<\/jats:sub>2.3<jats:sup>+\/\u2212<\/jats:sup> mice whereas absolute latencies remained unchanged. In summary, our results demonstrate that Ca<jats:sub>v<\/jats:sub>2.3 VGCCs are mandatory for physiological auditory information processing in the ascending auditory tract.<\/jats:p>","DOI":"10.1111\/ejn.14591","type":"journal-article","created":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T16:28:03Z","timestamp":1570811283000},"page":"1583-1604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Functional implications of Ca<sub>v<\/sub>2.3 R\u2010type voltage\u2010gated calcium channels in the murine auditory system \u2013 novel vistas from brainstem\u2010evoked response audiometry"],"prefix":"10.1111","volume":"51","author":[{"given":"Andreas","family":"Lundt","sequence":"first","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"So\u00f3s","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Seidel","sequence":"additional","affiliation":[{"name":"Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Henseler","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Cognitive Neurophysiology Department of Psychiatry and Psychotherapy and University Hospital Cologne Faculty of Medicine University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varun","family":"Raj Ginde","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Imran Arshaad","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Ehninger","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Cognition, German Center for Neurodegenerative Diseases, (Deutsches Zentrum f\u00fcr Neurodegenerative Erkrankungen, DZNE) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Hescheler","sequence":"additional","affiliation":[{"name":"Institute of Neurophysiology Faculty of Medicine University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agapios","family":"Sachinidis","sequence":"additional","affiliation":[{"name":"Institute of Neurophysiology Faculty of Medicine University of Cologne Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl","family":"Broich","sequence":"additional","affiliation":[{"name":"Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carola","family":"Wormuth","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Papazoglou","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-4752","authenticated-orcid":false,"given":"Marco","family":"Weiergr\u00e4ber","sequence":"additional","affiliation":[{"name":"Experimental Neuropsychopharmacology Federal Institute for Drugs and Medical Devices (Bundesinstitut f\u00fcr Arzneimittel und Medizinprodukte, BfArM) Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2014.00029"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-007-0091-x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/elm008"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00243-4"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.12.017"},{"volume-title":"Biology of the NMDA receptor","year":"2009","author":"Bloodgood B. L.","key":"e_1_2_10_7_1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-10-104"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-34-10832.2003"},{"volume-title":"Auditory evoked potentials: basic principles and clinical application","year":"2007","author":"Burkard R. F.","key":"e_1_2_10_10_1"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.5"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010986303924"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2011.01.016"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00430-6"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-003-4020-3"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl355"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000059243"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3468-14.2016"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813213106"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.2918"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-22-08715.2001"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(03)00054-6"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705756104"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01198"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-2-r19"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(03)00401-5"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.08.020"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005686"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.4161\/chan.24104"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.01.058"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-007-0098-3"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.145219"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.074740"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467208666150507104937"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00948.2011"},{"key":"e_1_2_10_36_1","first-page":"1011","article-title":"A new method for analyzing auditory brain\u2010stem response waveforms using a moving\u2010minimum subtraction procedure of digitized analog recordings","volume":"10","author":"Kallstrand J.","year":"2014","journal-title":"Neuropsychiatric Disease and Treatment"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510197200"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.249482"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00536.2006"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2013.08.002"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101469200"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0013-9580.2005.35304.x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:27:2:225"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2011.05.010"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1159\/000076922"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.04.024"},{"key":"e_1_2_10_47_1","first-page":"1","article-title":"Data acquisition and analysis in brainstem evoked response audiometry in mice","volume":"147","author":"Lundt A.","year":"2019","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2097-12.2012"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2003.00383.x"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76681-1"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05605.x"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.053256"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.883"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.12.032"},{"key":"e_1_2_10_55_1","first-page":"2","article-title":"Hearing loss: Does gender play a role?","volume":"2","author":"Murphy M. P.","year":"1997","journal-title":"Medscape Womens Health"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.05.057"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.90707.2008"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00030.2017"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1142-12.2012"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.412231"},{"key":"e_1_2_10_61_1","first-page":"385","article-title":"[Subunit of voltage\u2010dependent calcium channels in the murine spiral ganglion cells]","volume":"39","author":"Peng H.","year":"2004","journal-title":"Zhonghua Er Bi Yan Hou Ke Za Zhi"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.16.4.884"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2002"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1617533114"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00013-1"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080408"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00669.x"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201110699"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3005-12.2012"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2006.12.011"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.5665\/sleep.3652"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8388125"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00689.x"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050308"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(95)00079-J"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1393"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1009-06.2006"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(93)90140-D"},{"key":"e_1_2_10_79_1","first-page":"1115","article-title":"Localization of the calcium channel subunits Cav1.2 (alpha1C) and Cav2.3 (alpha1E) in the mouse organ of Corti","volume":"18","author":"Waka N.","year":"2003","journal-title":"Histology and Histopathology"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2006.00541.x"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01193.2006"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-004-0488-1"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2006.07.004"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2009.12.005"},{"key":"e_1_2_10_85_1","doi-asserted-by":"crossref","first-page":"22347","DOI":"10.1016\/S0021-9258(17)31796-9","article-title":"Structure and functional characterization of neuronal alpha 1E calcium channel subtypes","volume":"269","author":"Williams M. E.","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-23-08566.2000"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-02-00726.1999"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-16-05944.2001"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00042.2004"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1112"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-10-06419.1995"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03810.x"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.02.042"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.251272"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.181917"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2541-10.2010"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fejn.14591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.14591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ejn.14591","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.14591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T21:38:48Z","timestamp":1694036328000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.14591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,3]]},"references-count":95,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1111\/ejn.14591"],"URL":"https:\/\/doi.org\/10.1111\/ejn.14591","archive":["Portico"],"relation":{},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"type":"print","value":"0953-816X"},{"type":"electronic","value":"1460-9568"}],"subject":[],"published":{"date-parts":[[2019,11,3]]},"assertion":[{"value":"2018-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}