{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:42:49Z","timestamp":1791726169485,"version":"4.3.4"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fibroblast growth factor 23 (FGF23) is an endocrine growth factor and known to play a pivotal role in phosphate homeostasis. Interestingly, several studies point towards a function of FGF23 in the hypothalamus. FGF23 classically activates the FGF receptor 1 in the presence of the co\u2010receptor \u03b1Klotho, of both gene expression in the brain was previously established. However, studies on gene and protein expression of FGF23 in the brain are scarce and have been inconsistent. Therefore, our aim was to localise FGF23 gene and protein expression in the rat brain with focus on the hypothalamus. Also, we investigated the protein expression of \u03b1Klotho. Adult rat brains were used to localise and visualise FGF23 and \u03b1Klotho protein in the hypothalamus by immunofluorescence labelling. Furthermore, western blots were used for assessing hypothalamic FGF23 protein expression. FGF23 gene expression was investigated by qPCR in punches of the arcuate nucleus, lateral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for FGF23 and \u03b1Klotho protein was found in the hypothalamus, third ventricle lining and the choroid plexus. Western blot analysis of the hypothalamus confirmed the presence of FGF23. Gene expression of FGF23 was not detected, suggesting that the observed FGF23 protein is not brain\u2010derived. Several FGF receptors are known to be present in the brain. Therefore, we conclude that the machinery for FGF23 signal transduction is present in several brain areas, indeed suggesting a role for FGF23 in the brain.<\/jats:p>","DOI":"10.1111\/ejn.15375","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T01:16:49Z","timestamp":1624929409000},"page":"5261-5271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Localization of fibroblast growth factor 23 protein in the rat hypothalamus"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9989-0821","authenticated-orcid":false,"given":"Stan R.","family":"Ursem","sequence":"first","affiliation":[{"name":"Endocrine Laboratory, Department of Clinical Chemistry, Amsterdam Gastroenterology &amp; Metabolism Amsterdam UMC, Vrije Universiteit Amsterdam and University of Amsterdam  Amsterdam The 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Arts and Sciences (KNAW)  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Unga A.","family":"Unmehopa","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"},{"name":"Metabolism and Reward Group, Netherlands Institute for Neuroscience An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW)  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Eggels","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience Amsterdam UMC, University of Amsterdam  Amsterdam The Netherlands"},{"name":"Metabolism and Reward Group, Netherlands Institute for Neuroscience An 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