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To test the\n                    <jats:italic>in vivo<\/jats:italic>\n                    impact of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    increment in dopaminergic neurons physiology, we downregulated the plasma membrane Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ATPase (PMCA), a pump that extrudes cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , by expressing PMCA\n                    <jats:sup>RNAi<\/jats:sup>\n                    in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    <\/jats:styled-content>\n                    dopaminergic neurons. In these animals, we observed major locomotor alterations paralleled to higher cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and increased levels of oxidative stress in mitochondria. Interestingly, although no overt degeneration of dopaminergic neurons was observed, evidences of neuronal dysfunctionality were detected such as increases in presynaptic vesicles in dopaminergic neurons and in the levels of dopamine in the brain, as well as presence of toxic effects when PMCA was downregulated in the eye. Moreover, reduced PMCA levels were found in a\n                    <jats:italic>Drosophila<\/jats:italic>\n                    model of Parkinson's disease, Parkin knock\u2010out, expanding the functional relevance of PMCA reduction to other Parkinson's disease\u2010related models. In all, we have generated a new model to study motor abnormalities caused by increments in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    that lead to augmented oxidative stress in a dopaminergic environment, added to a rise in synaptic vesicles and dopamine levels.\n                  <\/jats:p>","DOI":"10.1111\/ejn.15401","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T23:32:35Z","timestamp":1627342355000},"page":"5915-5931","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Plasma membrane calcium ATPase downregulation in dopaminergic neurons alters cellular physiology and motor behaviour in\n                    <scp>\n                      <i>Drosophila melanogaster<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"54","author":[{"given":"Brenda","family":"Erhardt","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones 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