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The specific mechanisms underlying SCI\u2010induced neuropathic pain (SCI\u2010NP) remain unclear, and protein biomarkers have not yet been integrated into diagnostic screening. To better understand the host molecular pathways involved in SCI\u2010NP, we used the bioinformatics method, the PubMed database and bioinformatics methods to identify target genes and their associated pathways. We reviewed 2504 articles on the regulation of SCI\u2010NP and used the text mining of PubMed database abstracts to determine associations among 12 pathways and networks. Based on this method, we identified two central genes in SCI\u2010NP: interleukin\u20106 (IL\u20106) and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1). Adult male Sprague\u2013Dawley rats were used to build the SCI\u2010NP models. The threshold for paw withdrawal was significantly reduced in the SCI group, and TLR4 was activated in microglia after SCI. Enzyme\u2010linked immunosorbent assay\uff08ELISA) analysis of TNF\u2010\u03b1 and IL\u20106 levels was significantly higher in the SCI group than in the sham group. Western blot showed that expressions of the TLR4\/MyD88\/NF\u2010\u03baB inflammatory pathway protein increased dramatically in the SCI group. Using the TLR4 inhibitor TAK\u2010242, the pain threshold and expressions of inflammatory factors and proteins of the proteins of the inflammatory signal pathway were reversed, TLR4 in microglia was suppressed, suggesting that SCI\u2010NP was related to neuroinflammation mediated by the TLR4 signalling pathway. In conclusion, we found that TNF\u2010\u03b1 and IL\u20106 were the neuroinflammation\u2010related genes involved in SCI\u2010NP that can be alleviated by inhibiting the inflammatory pathway upstream of the TLR4\/MyD88\/NF\u2010\u03baB inflammatory pathway.<\/jats:p>","DOI":"10.1111\/ejn.15693","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T01:43:00Z","timestamp":1652406180000},"page":"3991-4008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Systematic analysis of critical genes and pathways identified a signature of neuropathic pain after spinal cord injury"],"prefix":"10.1111","volume":"56","author":[{"given":"Zefu","family":"Li","sequence":"first","affiliation":[{"name":"Department of Basic Medical College Human Anatomy of Zhengzhou University  Zhengzhou 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