{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:55:13Z","timestamp":1792122913190,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["SFI\/12\/RC\/2273_P2"],"award-info":[{"award-number":["SFI\/12\/RC\/2273_P2"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"publisher","award":["SFI21\/PATH\u2010S\/9424"],"award-info":[{"award-number":["SFI21\/PATH\u2010S\/9424"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The gut microbiota communicates with the brain through several pathways including the vagus nerve, immune system, microbial metabolites and through the endocrine system. Pathways along the humoral\/immune gut microbiota\u2013brain axis are composed of a series of vascular and epithelial barriers including the intestinal epithelial barrier, gut\u2013vascular barrier, blood\u2013brain barrier and blood\u2013cerebrospinal fluid barrier. Of these barriers, the relationship between the gut microbiota and blood\u2013cerebrospinal fluid barrier is yet to be fully defined. Here, using a germ\u2010free mouse model, we aimed to assess the relationship between the gut microbiota and the integrity of the blood\u2013cerebrospinal fluid barrier, which is localized to the choroid plexus epithelium. Using confocal microscopy, we visualized the tight junction protein zonula occludens\u20101, an integral aspect of choroid plexus integrity, as well as the choroid plexus fenestrated capillaries. Quantification of tight junction proteins via network analysis led to the observation that there was a decrease in the zonula occludens\u20101 network organization in germ\u2010free mice; however, we did not observe any differences in capillary structure. Taken together, these data indicate that the blood\u2013cerebrospinal fluid barrier is another barrier along the gut microbiota\u2013brain axis. Future studies are required to elucidate its relative contribution in signalling from microbiota to the brain.<\/jats:p>","DOI":"10.1111\/ejn.15878","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T08:12:33Z","timestamp":1669882353000},"page":"233-241","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["The gut microbiota is important for the maintenance of blood\u2013cerebrospinal fluid barrier integrity"],"prefix":"10.1111","volume":"57","author":[{"given":"Emily G.","family":"Knox","sequence":"first","affiliation":[{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"},{"name":"Pharmacodelivery Group, School of Pharmacy University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caoimhe M. K.","family":"Lynch","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"},{"name":"Department of Anatomy and Neuroscience University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye Seul","family":"Lee","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"},{"name":"Department of Anatomy and Neuroscience University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitriona M.","family":"O'Driscoll","sequence":"additional","affiliation":[{"name":"Pharmacodelivery Group, School of Pharmacy University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Clarke","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"},{"name":"Department of Psychiatry and Neurobehavioural Science University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Cryan","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"},{"name":"Department of Anatomy and Neuroscience University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5806-251X","authenticated-orcid":false,"given":"Maria R.","family":"Aburto","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork  Cork Ireland"},{"name":"Department of Anatomy and Neuroscience University College Cork  Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009759"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0006-15.2015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3346"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2018"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2021.02.007"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2017.12.010"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ame2.12257"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2018.1479625"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32366-6"},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","unstructured":"Hoyles L. Pontifex M. G. Rodriguez\u2010Ramiro I. Anis\u2010Alavi M. A. Jelane K. S. Snelling T. Solito E. Fonseca S. Carvalho A. L. Carding S. R. M\u00fcller M. Glen R. C. Vauzour D. &McArthur S.(2021). 'Regulation of blood\u2013brain barrier integrity and cognition by the microbiome\u2010associated methylamines trimethylamine\u2010N\u2010oxide and trimethylamine' Preprint.","DOI":"10.1101\/2021.01.28.428430"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0439-y"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2018.06.033"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00392"},{"key":"e_1_2_10_15_1","doi-asserted-by":"crossref","unstructured":"Knox E. G. Aburto M. R. Tessier C. Nagpal J. Clarke G. O'Driscoll C. M. andCryan J. F.(2022). 'Microbial\u2010derived metabolites induce actin cytoskeletal rearrangement and protect blood\u2010brain barrier function' iScience.","DOI":"10.1016\/j.isci.2022.105648"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-022-01511-z"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2020.09.027"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3921"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-022-00323-1"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00460-0"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2022.111572"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2019.1638722"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2014.01.159"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40263-016-0370-3"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-020-00196-2"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.100"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddmod.2019.08.002"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2016.1154641"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-018-0102-9"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2017.188"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S254403"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.06.118"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.832672"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.15878","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ejn.15878","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.15878","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T02:56:36Z","timestamp":1692500196000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.15878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,9]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/ejn.15878"],"URL":"https:\/\/doi.org\/10.1111\/ejn.15878","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/EJN.15878\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.15878\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.15878\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.15878\/v2\/response1","asserted-by":"object"}]},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,9]]},"assertion":[{"value":"2022-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}