{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:54:08Z","timestamp":1777949648608,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to explore the interaction between the expression of neuronal HIF\u20101\u03b1 in the mediobasal hypothalamus and food intake, glycolipid metabolism and body weight (BW) in mice consuming high\u2010fat diet (HFD). In HIF\u20101\u03b1\n                    <jats:sup>\n                      <jats:italic>flox\/flox<\/jats:italic>\n                    <\/jats:sup>\n                    mice, AAV\u2010hSyn\u2010GFP (NC group) or AAV\u2010hSyn\u2010cre\u2010GFP (KD group) virus was injected into medial base of the hypothalamus. Frozen brain tissue sections confirmed the presence of the virus within the hypothalamus of mice after 28\u2009days of AAV injection, including reporter signals within the arcuate nucleus, dorsomedial hypothalamic nucleus (DMH) and ventromedial hypothalamus (VMH). Consistently, the levels of HIF\u20101\u03b1 mRNA in the ventral hypothalamus were significantly lower in the KD group compared to the NC group. These KD mice also demonstrated significantly increased food intake, body weight (BW), total cholesterol (TC), high\u2010density lipoprotein (HDL), low\u2010density lipoprotein (LDL) and serum insulin, combined with higher blood glucose, compared to NC animals. However, the levels of triglycerides and FFA were similar in both groups. Significant differences in p\u2010Akt levels were not observed in the skeletal muscle, liver or epididymal fat in KD mice after insulin injection. In conclusion, the knockdown of HIF\u20101\u03b1 within the neurons of mediobasal hypothalamus results in an increase in the appetite of mice fed with HFD, which in turn leads to a significant dysregulation of lipid and glucose metabolism and a corresponding increase in weight. Therefore, the neuronal HIF\u20101\u03b1 expression in the mediobasal hypothalamus may be a critical regulator of glycolipid metabolism and body weight control when a high\u2010fat diet is consumed.\n                  <\/jats:p>","DOI":"10.1111\/ejn.16583","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T02:55:34Z","timestamp":1733453734000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neuronal HIF\u20101\u03b1 expression in mediobasal hypothalamus affects glycolipid metabolism and body weight in mice fed with high\u2010fat diet"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1700-9004","authenticated-orcid":false,"given":"Lijuan","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Physiology, School of Basic Medical Sciences Xinjiang Medical University  Urumqi Xinjiang China"},{"name":"Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases  Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azmat","family":"Rozjan","sequence":"additional","affiliation":[{"name":"Xinjiang Deyuan Bioengineering Co., Ltd, Urumqi  Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dilihumaier","family":"Duolikun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Physiology, School of Basic Medical Sciences Xinjiang Medical University  Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Physiology, School of Basic Medical Sciences Xinjiang Medical University  Urumqi Xinjiang China"},{"name":"Department of Anesthesiology The First Affiliated Hospital of Xinjiang Medical University  Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoling","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Physiology, School of Basic Medical Sciences Xinjiang Medical University  Urumqi Xinjiang China"},{"name":"Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases  Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-021-01710-0"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2021.12.008"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0000000000000597"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b01253"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.14280"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2018.06.020"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/abr.abr_105_20"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/1028415X.2018.1465713"},{"key":"e_1_2_12_10_1","unstructured":"HuangX.(2021).Intestinal epithelial HIF\u20101\u03b1 regulates intestinal mucosal barrier function in high\u2010fat diet fed mice. 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