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Considering the importance of 5\u2010HT1A receptors in modulating both pain and raphe neuronal activity, in this study, we aimed to determine whether 5\u2010HT1A\u2010dependent visceral and somatic nociceptive processing within the RMg and DR is modified in postcolitis conditions. In anaesthetised male Wistar rats, healthy control and recovered from TNBS\u2010induced colitis, the microelectrode recordings of RMg and DR neuron responses to noxious colorectal distension (CRD) or tail squeezing (TS) were performed prior and after intravenous administration of 5\u2010HT1A agonist, buspirone. In postcolitis animals, 5\u2010HT1A autoreceptor\u2010 and heteroreceptor\u2010activating high doses of buspirone (2 and 4\u2009mg\/kg) lost normally occurring ability to facilitate CRD\u2010 and TS\u2010evoked activation of RMg neurons, causing inhibition of the local nociceptive signalling similar to 5\u2010HT1A autoreceptor\u2010activating low doses (0.1 and 0.5\u2009mg\/kg). Conversely, the normally inherent property of buspirone at all doses to reduce visceral and somatic pain\u2013related neuronal excitation in the DR was weakened after colitis. These phenomena were associated with a loss of normally occurring inhibitory effect of the compound's high doses on hemodynamic reactions to CRD and TS, revealing deficient antinociceptive action at a systemic level. The data suggest postcolitis changes in buspirone\u2010dependent 5\u2010HT1A autoreceptor\u2010 and heteroreceptor\u2010mediated signalling, which can directly or indirectly lead to reduced RMg pain\u2013related activity and increased DR nociceptive excitation, impairing their functioning in the visceral and somatic pain control.<\/jats:p>","DOI":"10.1111\/ejn.16677","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T05:53:36Z","timestamp":1737352416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Postcolitis Alterations in Dose\u2010Dependent Effects of 5\u2010HT1A Agonist Buspirone on Nociceptive Activity of the Raphe Magnus and Dorsal Raphe Neurons in Rats"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-4628","authenticated-orcid":false,"given":"Olga\u00a0A.","family":"Lyubashina","sequence":"first","affiliation":[{"name":"Laboratory of Cortico\u2010Visceral Physiology Pavlov Institute of Physiology of the Russian Academy of Sciences  Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1359-439X","authenticated-orcid":false,"given":"Boris\u00a0M.","family":"Sushkevich","sequence":"additional","affiliation":[{"name":"Laboratory of Cortico\u2010Visceral Physiology Pavlov Institute of Physiology of the Russian Academy of Sciences  Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-8823","authenticated-orcid":false,"given":"Ivan\u00a0B.","family":"Sivachenko","sequence":"additional","affiliation":[{"name":"Laboratory of Cortico\u2010Visceral Physiology Pavlov Institute of Physiology of the Russian Academy of Sciences  Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,20]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","article-title":"Effect of Buspirone on Inflammation, Pain and Gastric Injury in Mice","volume":"6","author":"Abdel Salam O.","year":"2007","journal-title":"International Journal of Pharmacology"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abo0689"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddmec.2006.09.001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53552"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1007672008297"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028\u20103908(80)90187\u20102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.1998.tb10192.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2005.01.019"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1300061"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00825.2002"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40263\u2010013\u20100071\u20100"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21\u201024\u201009917.2001"},{"issue":"12","key":"e_1_2_11_14_1","first-page":"5580","article-title":"Activation of 5\u2010HT and NR2B Contributes to Visceral Hypersensitivity in Irritable Bowel Syndrome in Rats","volume":"8","author":"Chen M. 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