{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T15:05:47Z","timestamp":1780499147895,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T00:00:00Z","timestamp":1754524800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01 NS114480"],"award-info":[{"award-number":["R01 NS114480"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["RO1 AA027111"],"award-info":[{"award-number":["RO1 AA027111"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alzheimer's disease (AD) is the most common age\u2010related dementia. Lifestyle factors, including alcohol use, can increase the risk of AD. While human studies demonstrate that alcohol use can negatively impact AD risk and disease progression, the underlying ethanol\u2010dependent mechanisms that increase A\u03b2 burden and impair cognition remain elusive. We have recently shown that ethanol can acutely affect microglial dynamics, which are critical to microglial function, and many other studies have reported inflammatory activation of microglia after long\u2010term ethanol exposure in both humans and animal models. Here, we administered 4\u2009weeks of ethanol at dosages that mimic human binge drinking to 2.5\u2010month\u2010old male 5xF\n                    <jats:sc>AD<\/jats:sc>\n                    mice, a common mouse model of AD. After a 2\u2010week abstinence period, we performed behavioral assays and analyzed amyloid pathology and microglial morphology in the subiculum where amyloid pathology develops earlier than in other brain regions. We also analyzed the microglial transcriptome in the hippocampus. We found that ethanol exposure facilitated amyloid pathology and worsened cognitive function in 5xFAD mice, while microglial expression patterns, arborization, and phagocytosis appeared unchanged. Overall, our results suggest that pronounced ethanol exposure, when started early in the disease before amyloid pathology is established, can worsen AD progression in an amyloidosis model.\n                  <\/jats:p>","DOI":"10.1111\/ejn.70218","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T03:55:44Z","timestamp":1754625344000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ethanol Increases Diffuse Amyloid Plaque Load and Impairs Memory in the 5xFAD Mouse Model of Alzheimer's Disease"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2762-7044","authenticated-orcid":false,"given":"Linh","family":"Le","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Del Monte Institute for Neuroscience University of Rochester  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca\u00a0L.","family":"Lowery","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Del Monte Institute for Neuroscience University of Rochester  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Arya","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Del Monte Institute for Neuroscience University of Rochester  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1644-9767","authenticated-orcid":false,"given":"Florence\u00a0P.","family":"Varodayan","sequence":"additional","affiliation":[{"name":"Developmental Exposure Alcohol Research Center and Department of Psychology Binghamton University\u2010SUNY  Binghamton New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1246-3363","authenticated-orcid":false,"given":"M.\u00a0Kerry","family":"OBanion","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Del Monte Institute for Neuroscience University of Rochester  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2167-6849","authenticated-orcid":false,"given":"Ania\u00a0K.","family":"Majewska","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Del Monte Institute for Neuroscience University of Rochester  Rochester New York USA"},{"name":"Center for Visual Science University of Rochester  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22835"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13809"},{"key":"e_1_2_10_4_1","first-page":"50978","article-title":"Marble Burying and Nestlet Shredding as Tests of Repetitive, Compulsive\u2010Like Behaviors in Mice","volume":"82","author":"Angoa\u2010Perez M.","year":"2013","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.884170"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddaa038"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-018-0057-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2020.104956"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.836827"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7176"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2008.11.002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2022.105967"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-020-05589-7"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803590"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803590"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2021.112105"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1510329112"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2007.11.017"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)70016-5"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2017.03.006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567205013666160603005035"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.irn.2019.10.017"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.irn.2019.10.017"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-017-0703-3"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-00913-5"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1184-7"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2011.44.2.135"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/61.9.797"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144405"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2023.1230082"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12883"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.155"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2022.01.013"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2017.10.035"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.15225"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/neuroglia5030020"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1202-06.2006"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25126766"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2020.03.011"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-5"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-5-10"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.12.024"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-210523"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14706"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.05.003"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.70218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T19:45:43Z","timestamp":1755200743000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.70218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/ejn.70218"],"URL":"https:\/\/doi.org\/10.1111\/ejn.70218","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/EJN.70218\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70218\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70218\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70218\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70218\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70218\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70218\/v1\/review1","asserted-by":"object"}]},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2024-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70218"}}