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Little is known about the composition and function of Kv2 channels in spinal cord neurons, however. Here, we show that while Kv2.1 is broadly expressed in multiple classes of spinal cord neurons, the Kv6.4 \u201celectrically silent\u201d subunit is specifically expressed in motoneurons. In motoneurons, we find that Kv6.4 protein is coclustered with Kv2.1 and Kv2.2 subunits at endoplasmic reticulum\u2013plasma membrane (ER\u2010PM) junctions beneath C\u2010bouton synapses. In Kv2.1 S590A mutant mice, in which Kv2.1 is unable to bind ER VAP proteins, Kv2.1 and Kv6.4 clustering at ER\u2010PM junctions is severely reduced suggesting Kv2 channels are localized at ER\u2010PM junctions by the same molecular mechanism in motoneurons and brain neurons. Moreover, clustering of Kv6.4, as well as the AMIGO\u20101 auxiliary subunit, are severely reduced in Kv2.1 knockout mice and moderately reduced in Kv2.2 knockout mice. Thus, expression and localization of Kv6.4 subunits is dependent on Kv2 subunits, likely through their coassembly into heteromeric channels. Finally, we find that presynaptic C\u2010boutons and postsynaptic clusters of the ER\u2010resident sigma\u20101 receptor are preserved in motoneurons of Kv2 knockout mice. Together, these findings identify a specific Kv2\/KvS channel subtype expressed in motoneurons that localizes to C\u2010bouton junctions where it could regulate neuronal excitability and\/or signaling at ER\u2010PM junctions.\n                  <\/jats:p>","DOI":"10.1111\/ejn.70243","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T12:22:13Z","timestamp":1757334133000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Kv2\/Kv6.4 Heteromeric Potassium Channels Are Expressed in Spinal Motoneurons and Localized at C\u2010Bouton Synapses"],"prefix":"10.1111","volume":"62","author":[{"given":"Taylor\u00a0A.","family":"Lacey","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine University of California Davis  Davis California 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