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To test whether previous stress\u2010induced anxiety is associated with neuronal adaptations in the PBN (CGRP)\u2009\u2192\u2009BNST circuit, C57 mice were exposed to 4\u2009days of daily forced swim stress (FSS). The novelty\u2010suppressed feeding test (NSFT) demonstrated repeated FSS increased anxiety\u2010like behavior. To assess if repeated FSS potentiates anxiety\u2010like behavior in mice with dysregulated affect, mice received chronic intermittent ethanol vapor followed by FSS in abstinence. Anxiety\u2010like behavior measured by NSFT was potentiated by repeated FSS in alcohol\u2010abstinence. To measure neuroadaptations associated with a history of stress,\n                    <jats:italic>Calca<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>CRE<\/jats:italic>\n                    <\/jats:sup>\n                    mice, with the Cre\u2010dependent hM3D(Gq) DREADDs, in the PBN and the GFP\u2010based Ca2+\u2009sensor GCaMP7f in the bed nucleus of the stria terminalis (BNST), were exposed to repeated FSS and anxiety\u2010like behavior measured with the NSFT. Weeks later, neurotransmission was measured by recording GCaMP7f fluorescence in ex\u00a0vivo BNST slices. hM3D(Gq) DREADDs in PBN (CGRP) projections were activated via bath application of clozapine\u2010n\u2010oxide. PBN (CGRP) projections increased GCaMP7f spike frequency in the BNST in na\u00efve conditions but not after a history of repeated FSS. Post\u2010activation of PBN (CGRP) projections decreased GCaMP frequency and amplitude in the BNST, which was potentiated by a history of repeated FSS. These findings extend the anxiogenic role of PBN (CGRP) neurons by demonstrating neurotransmission in the PBN (CGRP)\u2009\u2192\u2009BNST circuit is dysregulated after a stress\u2010induced anxiogenic state. Given the potential of CGRP\u2010related therapies, future studies will investigate the role of CGRP within the PBN\u2009\u2192\u2009BNST circuit in inducing anxiety\u2010related neuroadaptations.\n                  <\/jats:p>","DOI":"10.1111\/ejn.70268","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T03:12:04Z","timestamp":1762744324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ex Vivo Modulation of the BNST by Parabrachial CGRP Projections Is Decreased After a History of Stress\u2010Induced Anxiety"],"prefix":"10.1111","volume":"62","author":[{"given":"L.\u00a0S.","family":"Kayat","sequence":"first","affiliation":[{"name":"School of Medicine Vanderbilt University  Nashville Tennessee USA"},{"name":"Vanderbilt Brain Institute  Nashville Tennessee USA"},{"name":"Vanderbilt Center for Addiction Research  Nashville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Petersen","sequence":"additional","affiliation":[{"name":"School of Medicine Vanderbilt University  Nashville Tennessee USA"},{"name":"Vanderbilt Brain Institute  Nashville Tennessee USA"},{"name":"Vanderbilt Center for Addiction Research  Nashville Tennessee USA"},{"name":"Department of Molecular Physiology and Biophysics Vanderbilt University  Nashville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u00a0E.","family":"Van\u00a0Doorn","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Kentucky College of Pharmacy  Lexington Kentucky USA"},{"name":"College of Pharmacy University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.\u00a0P.","family":"Nabit","sequence":"additional","affiliation":[{"name":"School of Medicine Vanderbilt University  Nashville Tennessee USA"},{"name":"Vanderbilt Brain Institute  Nashville Tennessee USA"},{"name":"Vanderbilt Center for Addiction Research  Nashville Tennessee USA"},{"name":"Department of Pharmacology Vanderbilt University  Nashville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u00a0B.","family":"Tyree","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Kentucky College of Pharmacy  Lexington Kentucky USA"},{"name":"College of Pharmacy University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u00a0W.","family":"Centanni","sequence":"additional","affiliation":[{"name":"School of Medicine Vanderbilt University  Nashville Tennessee USA"},{"name":"Vanderbilt Brain Institute  Nashville Tennessee USA"},{"name":"Vanderbilt Center for Addiction Research  Nashville Tennessee USA"},{"name":"Department of Molecular Physiology and Biophysics Vanderbilt University  Nashville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6267-4196","authenticated-orcid":false,"given":"A.\u00a0A.","family":"Jaramillo","sequence":"additional","affiliation":[{"name":"School of Medicine Vanderbilt University  Nashville Tennessee USA"},{"name":"Vanderbilt Brain Institute  Nashville Tennessee USA"},{"name":"Vanderbilt Center for Addiction Research  Nashville Tennessee USA"},{"name":"Department of Molecular Physiology and Biophysics Vanderbilt University  Nashville Tennessee USA"},{"name":"Department of Pharmaceutical Sciences University of Kentucky College of Pharmacy  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2022.09.010"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2015.185"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59799"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-022-01442-x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4574"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25511"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12596"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-018-0257-8"},{"issue":"891","key":"e_1_2_11_10_1","article-title":"Parabrachial CGRP Neurons Establish and Sustain Aversive Taste Memories","volume":"100","author":"Chen J. 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