{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:34:36Z","timestamp":1790829276916,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The discovery of olfactory bulb glomeruli was reported by the neuroanatomist Camillo Golgi in 1875, but the number of glomeruli in a mammalian olfactory bulb remains unknown. The 1141 intact odorant receptor genes in the mouse genome are key determinants of the coalescence of axons of olfactory sensory neurons into glomeruli at recognizable positions in the olfactory bulb. Here, we devised a methodology for quantifying the numbers, sizes, and shapes of the glomeruli of mouse olfactory bulbs. Using serial two\u2010photon tomography, we generated complete image stacks of 12 mouse olfactory bulbs labeled immunohistochemically as whole mounts with antibodies against the glomerular marker VGLUT2. The mouse ages were postnatal days 14, 21, and 56. We manually segmented 33,137 glomeruli based on the VGLUT2\u2010immunoreactive signal. This manual segmentation resulted in complete empirical counts of the heterogeneously\u2010sized glomerular 3D objects instead of providing only statistical estimates. We counted a median of 2851 glomeruli per olfactory bulb at postnatal day 56, corresponding to 2.50 glomeruli per intact odorant receptor gene per olfactory bulb. For a 3D object of any shape, the effective diameter is defined as the diameter of a sphere that possesses the equivalent volume. We found that the effective diameters of the glomeruli are well modeled by a Gaussian distribution, with a median of 77.54\u2009\u03bcm at postnatal day 56. By quantifying glomerular shape with aspect\u2010length ratios and sphericity, we demonstrate that glomeruli do not conform to spherical shapes. We propose the descriptor \u201ctuberiform\u201d to encompass the diversity of glomerular shapes.<\/jats:p>","DOI":"10.1111\/ejn.70327","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T07:46:42Z","timestamp":1765266402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Glomeruli of the Mouse Olfactory Bulb: Numbers, Sizes, Shapes"],"prefix":"10.1111","volume":"62","author":[{"given":"Yu","family":"Weng","sequence":"first","affiliation":[{"name":"Max Planck Research Unit for Neurogenetics Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bolek","family":"Zapiec","sequence":"additional","affiliation":[{"name":"Max Planck Research Unit for Neurogenetics Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Paredes","sequence":"additional","affiliation":[{"name":"Max Planck Research Unit for Neurogenetics Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8082-4971","authenticated-orcid":false,"given":"Peter","family":"Mombaerts","sequence":"additional","affiliation":[{"name":"Max Planck Research Unit for Neurogenetics Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090940210"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u2010185X.1953.tb01376.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2016.00031"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200501726"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010020\u20106583\u20103"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.11.001"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22\u201008\u201003033.2002"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.11.010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23835"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(91)90418\u2010X"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(00)80698\u20104"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200501120"},{"key":"e_1_2_11_14_1","first-page":"133","article-title":"Origen y Terminaci\u00f3n de las Fibras Nerviosas Olfatorias","volume":"3","author":"Cajal S.","year":"1890","journal-title":"Gaceta Sanitaria de Barcelona"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102818.1983.tb04255.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v064.i04"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.05.013"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.05.011"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.10.046"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2013.05.002"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460\u20109568.2007.05400.x"},{"key":"e_1_2_11_22_1","first-page":"405","article-title":"Sulla Fina Struttura dei Bulbi Olfactorii","volume":"1","author":"Golgi C.","year":"1875","journal-title":"Rivista Sperimentale di Freniatria e Medicina Legale"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(86)90119\u20103"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.001"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2752\u201009.2009"},{"key":"e_1_2_11_26_1","volume-title":"A Primer on Partial Least Squares Structural Equation Modeling (PLS\u2010SEM)","author":"Hair J. 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B.Zapiec V.Blanz andP.Mombaerts.2024. \u201cA Weighted Consensus Deep Learning Method for the Fusion of Object Segmentation Models and Its Application to Glomeruli of the Mouse Olfactory Bulb.\u201d 2024 IEEE International Symposium on Biomedical Imaging (ISBI) 1\u20135.https:\/\/doi.org\/10.1109\/ISBI56570.2024.10635675.","DOI":"10.1109\/ISBI56570.2024.10635675"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1010209925783"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0123\u201016.2016"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awx208"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512135112"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.02.110"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.70327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T06:26:00Z","timestamp":1766730360000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.70327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":68,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/ejn.70327"],"URL":"https:\/\/doi.org\/10.1111\/ejn.70327","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/EJN.70327\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70327\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70327\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70327\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70327\/v1\/review2","asserted-by":"object"}]},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70327"}}