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Brain areas critical for sexual experience\u2013enhanced paced mating behavior are unknown. Here, designer receptors exclusively activated by designer drugs (DREADDs) were used to test the hypothesis that silencing the medial amygdala (MeA) or ventromedial nucleus of the hypothalamus (VMH) across four paced mating tests would disrupt not only the display of paced mating behavior but also the enhancement of mating with sexual experience. Sexually na\u00efve, ovariectomized rats received bilateral infusion into the MeA or VMH of a viral vector containing the gene for an inhibitory G\u2010protein\u2013coupled receptor (hM4Di) 3\u2009weeks before behavioral testing. Rats were hormone primed with estradiol benzoate\u2009+\u2009progesterone and received either 1\u2009mg\/kg i.p. clozapine n\u2010oxide (CNO) or vehicle 30\u2009min before each of four 15\u2010intromission tests of paced mating behavior. CNO given to rats lacking DREADD infusions did not exhibit disruptions to paced mating behavior. CNO\u2010induced inhibition of the MeA altered paced mating behavior beginning with the first test, pointing to a role for the MeA in sensory processing of sexual stimulation. In contrast, inhibition of the VMH only led to changes in paced mating behavior on Tests 2\u20134, indicating that neural plasticity is induced in the VMH by sexual experience.<\/jats:p>","DOI":"10.1111\/ejn.70445","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:14:59Z","timestamp":1772979299000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DREADD\u2010Induced Inhibition of the Medial Amygdala and Ventromedial Nucleus of the Hypothalamus Affects Experience\u2010Induced Enhancement of Paced Mating Behavior in Female Rats"],"prefix":"10.1111","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2526-1955","authenticated-orcid":false,"given":"Sarah\u00a0H.","family":"Meerts","sequence":"first","affiliation":[{"name":"Neuroscience Program and Department of Psychology Carleton College  Northfield Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antao","family":"Shao","sequence":"additional","affiliation":[{"name":"Neuroscience Program and Department of Psychology Carleton College  Northfield Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabrielle\u00a0L.","family":"Reynaga","sequence":"additional","affiliation":[{"name":"Neuroscience Program and Department of Psychology Carleton College  Northfield Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia\u00a0H.","family":"d'Auria","sequence":"additional","affiliation":[{"name":"Neuroscience Program and Department of Psychology Carleton College  Northfield Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2013.10.003"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700293104"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.1.4986"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4530(84)90002-7"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1286.023"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1635-23.2024"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(88)90005-0"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903600203"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000122231"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-10-03843.1998"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(81)90228-6"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(96)00763-9"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.1996.04432.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90021-M"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(84)90019-9"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(89)90037-8"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1037\/0735-7044.106.4.690"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.1997.00660.x"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2004.01.026"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2008.07.011"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91365-X"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11930-017-0102-9"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2003.10.056"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1037\/h0032536"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.07.071"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2021.113206"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/endocr\/bqaa150"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2014.09.009"},{"key":"e_1_2_12_30_1","first-page":"109","article-title":"How Does Vaginal Stimulation Produce Pleasure, Pain, and Analgesia?","volume":"17","author":"Komisaruk B. 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