{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:22:46Z","timestamp":1786116166054,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001782","name":"University of Melbourne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001782","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004752","name":"State Government of Victoria","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004752","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>MicroRNAs (miRNAs) play crucial roles in regulating gene expression and have been implicated in the pathophysiology of depression. Among them, miR\u2010124\u20103p is one of the most abundant brain\u2010enriched miRNAs and has emerged as a potential therapeutic target for mood disorders. This study aimed to evaluate the antidepressant\u2010like effects of an acute administration of miR\u2010124\u20103p and to identify associated molecular and neuronal changes across key brain regions. Female C57BL\/6 mice received intracerebroventricular injections of a miR\u2010124\u20103p mimic or a control oligonucleotide. Affective\u2010like behavioural responses were assessed using the Porsolt swim test (PST) and the light\u2013dark box test (LDB). Proteomic alterations in the hippocampus, hypothalamus and prefrontal cortex were analysed by liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS), and stress\u2010related neuronal activity was evaluated using c\u2010Fos immunofluorescence. Mice treated with the miR\u2010124\u20103p mimic exhibited reduced immobility in the PST, indicative of an antidepressant\u2010like effect and enhanced active stress coping. Proteomic profiling revealed region\u2010specific changes, including downregulation of immune\u2010 and stress\u2010related proteins in the hypothalamus and hippocampus, and upregulation of synaptic and metabolic proteins in the prefrontal cortex. c\u2010Fos analyses showed decreased stress\u2010induced neuronal activation in the hypothalamic paraventricular and periventricular nuclei, alongside increased activity in the hippocampal dentate gyrus. Collectively, these findings suggest that miR\u2010124\u20103p mimic treatment is associated with antidepressant\u2010like effects by reducing neuroimmune activation and enhancing neuronal plasticity. Our results support a model in which miR\u2010124\u20103p promotes stress resilience through coordinated modulation of inflammatory and plasticity\u2010related processes across distinct brain circuits.<\/jats:p>","DOI":"10.1111\/ejn.70634","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T03:04:49Z","timestamp":1784516689000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting miR\u2010124\u20103p to Induce Antidepressant\u2010Like Effects in Female Mice: MicroRNA Modulation of Stress Resilience"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9646-0121","authenticated-orcid":false,"given":"Maria A.","family":"Kuznetsova","sequence":"first","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre University of Melbourne  Parkville Australia"},{"name":"School of Biomedical Sciences and Pharmacy The University of Newcastle  Callaghan Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5349-0080","authenticated-orcid":false,"given":"Alexandra","family":"Fraser","sequence":"additional","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Seng","family":"Ang","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Dentistry and Health Sciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Williamson","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Dentistry and Health Sciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nafisa","family":"Kazi","sequence":"additional","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7532-8922","authenticated-orcid":false,"given":"Anthony J.","family":"Hannan","sequence":"additional","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre University of Melbourne  Parkville Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9262-3971","authenticated-orcid":false,"given":"Thibault","family":"Renoir","sequence":"additional","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre University of Melbourne  Parkville Australia"},{"name":"Faculty of Medicine, Dentistry and Health Sciences University of Melbourne  Parkville Australia"},{"name":"Academic Quality Department Melbourne Polytechnic  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,19]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2010.08.023"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2017.45"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.9740\/mhc.2020.11.326"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.5607\/en.2015.24.2.156"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-023-02309-3"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23842"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2022.114154"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4050"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2018.07.075"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20190186"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2003.07.001"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0319-16.2016"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2003.09.011"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.270414"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3879"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S204461"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-023-02085-0"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354478"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1184-06.2006"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2019.101470"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073385"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2015.5"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2591-0"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-020-00964-4"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.31887\/DCNS.2019.21.4\/ebinder"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1301574"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2266"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2016.175"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22389"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086469"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10287"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2019.101732"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b16-00898"},{"key":"e_1_2_13_35_1","volume-title":"Depression and Other Common Mental Disorders Global Health Estimates","author":"WHO","year":"2017"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/0269881118758304"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-022-06524-2"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.4088\/JCP.20m13699"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2715325"}],"container-title":["European Journal of Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.70634","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ejn.70634","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ejn.70634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T08:22:56Z","timestamp":1786004576000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ejn.70634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/ejn.70634"],"URL":"https:\/\/doi.org\/10.1111\/ejn.70634","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/EJN.70634\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/EJN.70634\/v2\/review1","asserted-by":"object"}]},"ISSN":["0953-816X","1460-9568"],"issn-type":[{"value":"0953-816X","type":"print"},{"value":"1460-9568","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2025-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70634"}}