{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T08:57:12Z","timestamp":1781945832945,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,5,22]],"date-time":"2018-05-22T00:00:00Z","timestamp":1526947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bsssjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Soil Science"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec>\n                    <jats:title>Summary<\/jats:title>\n                    <jats:p>\n                      The origin and fate of humus, a soil organic matter fraction that is most resistant to decomposition, are not yet completely understood. Humus derives mainly from plant structural components; that is, lignin and cellulose, together with several other primary and secondary compounds. There are several methods to estimate lignin and cellulose in plant material; however, the applications to soil are limited and usually complicated and expensive. Conversely, humus can be estimated in soil by chemical titration methods, which estimate the carbon linked to humic and fulvic acids (HAC and FAC). Therefore, we carried out analyses that aimed to compare, within beech forest soils, spectrophotometric data for lignin and cellulose with those of HAC and FAC. We used soil samples from six different beech woods in Italy, sampling in four layers from 0\u20135 to 30\u201340\u2009cm. The relations between spectrophotometric cellulose and lignin and HAC and FAC were tested by linear mixed models. Our results showed that both lignin and cellulose were significant predictors for HAC and FAC, even if cellulose had a larger conditional coefficient of determination (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      <jats:sub>c<\/jats:sub>\n                      ). The best fit was between cellulose and the sum of HAC and FAC (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      <jats:sub>c<\/jats:sub>\n                      \u2009=\u20090.675). Our novel approach proved that spectrophotometric lignin and cellulose provide reliable results in both organic and mineral beech forest soils, even though lignin had a greater random variation than cellulose. Thus, spectrophotometric lignin and cellulose provide reliable and rapidly obtained predictors of humic substances in beech forest soils, with appropriate conversion factors to estimate HAC and FAC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Humic substances are of pivotal importance in the global carbon cycle.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>There is a need for simple and rapid methods to estimate humic substances.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Spectrophotometric methods provide fast results for cellulose and lignin in soils.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Lignin and cellulose function as good predictors for humic substances in forest soils.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ejss.12678","type":"journal-article","created":{"date-parts":[[2018,5,22]],"date-time":"2018-05-22T06:30:00Z","timestamp":1526970600000},"page":"856-867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Spectrophotometric methods for lignin and cellulose in forest soils as predictors for humic substances"],"prefix":"10.1111","volume":"69","author":[{"given":"T.","family":"Danise","sequence":"first","affiliation":[{"name":"Department of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fioretto","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2362-6025","authenticated-orcid":false,"given":"M.","family":"Innangi","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania \u201cLuigi Vanvitelli\u201d  Caserta Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,22]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"2157","article-title":"Impact of sole cropping and multiple cropping on soil humified carbon 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