{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T18:20:30Z","timestamp":1758478830840,"version":"3.37.3"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K07097"],"award-info":[{"award-number":["17K07097"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Euro J of Neurology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and purpose<\/jats:title><jats:p>To investigate prion protein (PrP) deposits in cutaneous tissues of patients of glycosylphosphatidylinositol (GPI)\u2010anchorless prion diseases with neuropathy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cutaneous tissue samples from three patients with GPI\u2010anchorless prion diseases were obtained, two cutaneous biopsy samples from the lower leg of Case 1 (Y162X) and Case 3 (D178fs25), and a cutaneous sample taken from the abdomen during an autopsy of Case 2 (D178fs25). We performed immunohistochemistry for PrP to look for abnormal PrP deposits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PrP deposits were observed in the dermal papilla, the sweat glands, the hair follicles, the arrector pili muscles, and peripheral nerves of all examined cases of GPI\u2010anchorless prion disease with neuropathy. The abnormal PrP accumulation was frequently localized at the basement membrane, and colocalized with laminin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Immunohistochemical detection of PrP in cutaneous samples could be used to definitively diagnose GPI\u2010anchorless PrP disease with neuropathy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ene.14720","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T04:17:04Z","timestamp":1610597824000},"page":"2133-2137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of cutaneous prion protein deposits could help diagnose GPI\u2010anchorless prion disease with neuropathy"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4225-7248","authenticated-orcid":false,"given":"Hiroyuki","family":"Honda","sequence":"first","affiliation":[{"name":"Department of Neuropathology Graduate School of Medical Sciences Kyushu University Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9455-3903","authenticated-orcid":false,"given":"Kosuke","family":"Matsuzono","sequence":"additional","affiliation":[{"name":"Division of Neurology Department of Medicine Jichi Medical University School of Medicine Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kota","family":"Satoh","sequence":"additional","affiliation":[{"name":"Department of Neurology Okayama University Graduate School of Medicine Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayoshi","family":"Fujisawa","sequence":"additional","affiliation":[{"name":"Department of Pathology and Experimental Medicine Graduate School for Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi O.","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Neuropathology Graduate School of Medical Sciences Kyushu University Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiaki","family":"Furuyama","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine Jichi Medical University School of Medicine Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuyuki","family":"Kitamoto","sequence":"additional","affiliation":[{"name":"Division of CJD Science and Technology Department of Neurological Science Tohoku University Graduate School of Medicine Sendai Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Division of Neurology Department of Medicine Jichi Medical University School of Medicine Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Neurology Okayama University Graduate School of Medicine Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Iwaki","sequence":"additional","affiliation":[{"name":"Department of Neuropathology Graduate School of Medical Sciences Kyushu University Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,22]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.1038\/nrneurol.2014.263"},{"doi-asserted-by":"publisher","key":"e_1_2_11_3_1","DOI":"10.1056\/NEJMoa1214747"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.1111\/ene.12089"},{"doi-asserted-by":"publisher","key":"e_1_2_11_5_1","DOI":"10.1093\/jnen\/nlw077"},{"doi-asserted-by":"publisher","key":"e_1_2_11_6_1","DOI":"10.1111\/ene.13665"},{"doi-asserted-by":"publisher","key":"e_1_2_11_7_1","DOI":"10.1002\/acn3.568"},{"doi-asserted-by":"publisher","key":"e_1_2_11_8_1","DOI":"10.1128\/JVI.00425-11"},{"doi-asserted-by":"publisher","key":"e_1_2_11_9_1","DOI":"10.1111\/j.1460-9568.2006.05156.x"},{"doi-asserted-by":"publisher","key":"e_1_2_11_10_1","DOI":"10.1086\/505914"},{"doi-asserted-by":"publisher","key":"e_1_2_11_11_1","DOI":"10.1016\/0006-8993(86)90602-5"}],"container-title":["European Journal of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ene.14720","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ene.14720","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ene.14720","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T01:12:20Z","timestamp":1693617140000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ene.14720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,22]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/ene.14720"],"URL":"https:\/\/doi.org\/10.1111\/ene.14720","archive":["Portico"],"relation":{},"ISSN":["1351-5101","1468-1331"],"issn-type":[{"type":"print","value":"1351-5101"},{"type":"electronic","value":"1468-1331"}],"subject":[],"published":{"date-parts":[[2021,1,22]]},"assertion":[{"value":"2020-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}