{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:13:00Z","timestamp":1759421580488,"version":"3.41.2"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Euro J of Neurology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and purpose<\/jats:title><jats:p>Myotonia congenita (MC) is a muscle channelopathy in which pathogenic variants in a key sarcolemmal chloride channel Gene (<jats:italic>CLCN1<\/jats:italic>) cause myotonia. This study used muscle magnetic resonance imaging (MRI) to quantify contractile properties and fat replacement of muscles in a Danish cohort of MC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Individuals with the Thomsen (dominant) and Becker (recessive) variants of MC were studied. Isometric muscle strength, whole\u2010body MRI, and clinical data were collected. The degree of muscle fat replacement of thigh, calf, and forearm muscles was quantitively calculated on Dixon MRI as fat fractions (FFs). Contractility was evaluated as the muscle strength per contractile muscle cross\u2010sectional area (PT\/CCSA). Muscle contractility was compared with clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Intramuscular FF was increased and contractility reduced in calf and in forearm muscles compared with controls (FF\u2009=\u20097.0\u201314.3% vs. 5.3\u20139.6%, PT\/CCSA\u2009=\u20091.1\u20134.9\u2009Nm\/cm<jats:sup>2<\/jats:sup> vs. 1.9\u20135.8\u2009Nm\/cm<jats:sup>2<\/jats:sup> [<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05]). Becker individuals also showed increased intramuscular FF and reduced contractility of thigh muscles (FF\u2009=\u200911.9% vs. 9.2%, PT\/CCSA\u2009=\u20091.9\u2009Nm\/cm<jats:sup>2<\/jats:sup> vs. 3.2\u2009Nm\/cm<jats:sup>2<\/jats:sup> [<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05]). Individual muscle analysis showed that increased FF was limited to seven of 18 examined muscles (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). There was a weak correlation between reduced contractility and severity of symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Individuals with MC have increased fat replacement and reduced contractile properties of muscles. Nonetheless, changes were small and likely did not impact clinically on their myotonic symptoms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ene.16207","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T13:29:19Z","timestamp":1706189359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Contractile properties and magnetic resonance imaging\u2010assessed fat replacement of muscles in myotonia congenita"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6886-7862","authenticated-orcid":false,"given":"Laura N\u00f8rager","family":"Jacobsen","sequence":"first","affiliation":[{"name":"Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mads Godtfeldt","family":"Stemmerik","sequence":"additional","affiliation":[{"name":"Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofie Vinther","family":"Skriver","sequence":"additional","affiliation":[{"name":"Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9496-7655","authenticated-orcid":false,"given":"Jonas Jalili","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoline","family":"L\u00f8kken","sequence":"additional","affiliation":[{"name":"Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Vissing","sequence":"additional","affiliation":[{"name":"Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nurt.2007.01.012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e32832efa8f"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/685328"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.26887"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-009-5049-y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.01404"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20295"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.2009.01228.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-015-8356-8"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp294"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-020-10328-1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2013.05.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2009.06.369"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-005-0684-4"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000006887"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000000851"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-29759-7"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000009828"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.28047"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-015-9798-5"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24743"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmr.2010.03.024"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000000828"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/2150131916659282"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2005.07.002"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000257819.47628.41"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.7863\/jum.1993.12.2.73"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-010-1799-2"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2018.07.001"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awt133"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000233841.75824.0f"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200810024"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15417"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2012.03751.x"}],"container-title":["European Journal of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ene.16207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T09:38:19Z","timestamp":1710754699000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ene.16207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,25]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/ene.16207"],"URL":"https:\/\/doi.org\/10.1111\/ene.16207","archive":["Portico"],"relation":{},"ISSN":["1351-5101","1468-1331"],"issn-type":[{"type":"print","value":"1351-5101"},{"type":"electronic","value":"1468-1331"}],"subject":[],"published":{"date-parts":[[2024,1,25]]},"assertion":[{"value":"2023-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e16207"}}