{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:38:42Z","timestamp":1791506322827,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,31]],"date-time":"2024-03-31T00:00:00Z","timestamp":1711843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Euro J of Neurology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and purpose<\/jats:title><jats:p>Antiganglioside antibodies (AGAs) might be involved in the etiopathogenesis of many neurological diseases, such as Miller\u2013Fisher syndrome (MFS) and Guillain\u2013Barr\u00e9 syndrome (GBS). Available comprehensive reference data regarding AGA positivity rates and cross\u2010responsiveness among AGAs (where one line immunoblot is positive for \u22651 AGA) during routine clinical care are scant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this 10\u2010year monocentric retrospective study, 3560 immunoglobulin (Ig) G and IgM line blots (GA Generic Assays' Anti\u2010Ganglioside Dot kit) obtained using cerebrospinal fluid (CSF) and serum samples from 1342 patients were analyzed for AGA positivity in terms of 14 diagnosis categories and AGA cross\u2010responsiveness.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of all 3560 line blots 158 (4.4%) and of all CSF samples 0.4% (4\/924) CSF line blots were AGA positive. For serum IgG, blots with positivity rates higher than the standard deviation of 15.6% were associated with MFS (GD3, GD1a, GT1a and GQ1b) and acute motor axonal neuropathy (AMAN) (GM1, GD1a and GT1a). For serum IgM, blots with positivity rates higher than the standard deviation of 8.1% were associated with AMAN (GM2, GT1a and GQ1b), MFS (GM1, GT1a and GQ1b), multifocal motor neuropathy (MMN) (GM1, GM2 and GQ1b) and chronic inflammatory demyelinating polyneuropathy (CIDP) (GM1). Cross\u2010responsiveness was observed in 39.6% of all positive serum AGA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Testing for AGAs during routine clinical care rarely led to positive findings, both in serum and even less in CSF, except for the diagnoses AMAN, MFS, MMN and CIDP. Nonspecific findings found as cross\u2010responsiveness between different AGA samples occur frequently, impacting the positivity of most AGA subtypes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ene.16290","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T04:56:04Z","timestamp":1711947364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antiganglioside antibody frequency in routine clinical care settings"],"prefix":"10.1111","volume":"31","author":[{"given":"Niklas","family":"Giesche","sequence":"first","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Tobias","family":"B\u00f6hm\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7575-0020","authenticated-orcid":false,"given":"Benedict","family":"Kleiser","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus C.","family":"Kowarik","sequence":"additional","affiliation":[{"name":"Department of Neurology and Stroke, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Dubois","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Stransky","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Armbruster","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8615-5040","authenticated-orcid":false,"given":"Alexander","family":"Grimm","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2039-5498","authenticated-orcid":false,"given":"Justus","family":"Marquetand","sequence":"additional","affiliation":[{"name":"Department of Epileptology, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"},{"name":"Department of Neural Dynamics and Magnetoencephalography, Hertie\u2010Institute for Clinical Brain Research University of T\u00fcbingen  T\u00fcbingen Germany"},{"name":"MEG\u2010Center University of T\u00fcbingen  T\u00fcbingen Germany"},{"name":"Institute for Modelling and Simulation of Biomechanical Systems  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,31]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm2.1935.235.1-2.24"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwl052"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.572965"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.5650\/jos.60.537"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1531-8249(199902)45:2<168::AID-ANA6>3.0.CO;2-6"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410310619"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440621"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410240113"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.34.1009"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214222"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402391101"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1086\/513800"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-20-2285"},{"issue":"2","key":"e_1_2_13_15_1","first-page":"117","article-title":"Comparison of immunoblotting and ELISA for detection of anti\u2010ganglioside antibodies in children with Guillain\u2013Barr\u00e9 syndrome","volume":"7","author":"Bonyadi MR","year":"2010","journal-title":"Iran J Immunol"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02301-0"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jns.12594"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.43.10.1911"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.43.2.414"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-008-0775-0"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2012-302824"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2015.02.039"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2004.01.005"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(99)00213-1"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(01)00465-8"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181ff94c2"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(01)00266-1"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756285615575269"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23881"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2007.09.020"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jns.12455"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2006.01161.x"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(93)90048-4"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000002558"}],"container-title":["European Journal of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ene.16290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T12:18:35Z","timestamp":1717762715000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ene.16290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,31]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/ene.16290"],"URL":"https:\/\/doi.org\/10.1111\/ene.16290","archive":["Portico"],"relation":{},"ISSN":["1351-5101","1468-1331"],"issn-type":[{"value":"1351-5101","type":"print"},{"value":"1468-1331","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,31]]},"assertion":[{"value":"2023-12-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e16290"}}