{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:30:30Z","timestamp":1781051430057,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["177966"],"award-info":[{"award-number":["177966"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["287842"],"award-info":[{"award-number":["287842"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004257","name":"Helse Vest","doi-asserted-by":"publisher","award":["29604"],"award-info":[{"award-number":["29604"]}],"id":[{"id":"10.13039\/501100004257","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004257","name":"Helse Vest","doi-asserted-by":"publisher","award":["911218"],"award-info":[{"award-number":["911218"]}],"id":[{"id":"10.13039\/501100004257","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013263","name":"Nasjonalforeningen for Folkehelsen","doi-asserted-by":"publisher","award":["14846"],"award-info":[{"award-number":["14846"]}],"id":[{"id":"10.13039\/501100013263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013263","name":"Nasjonalforeningen for Folkehelsen","doi-asserted-by":"publisher","award":["16152"],"award-info":[{"award-number":["16152"]}],"id":[{"id":"10.13039\/501100013263","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Euro J of Neurology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Alpha\u2010synuclein (\u03b1\u2010syn), phosphorylated at serine 129 (pS129\u2010\u03b1\u2010syn), is a potential biomarker for Parkinson's disease (PD) because it is the predominant \u03b1\u2010syn species found in Lewy bodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We developed an in\u2010house SIMOA assay, using commercially available components, to quantify pS129\u2010\u03b1\u2010syn in CSF. The clinical utility of the assay was tested in CSF from 120 patients with PD from the Norwegian ParkWest longitudinal study and 29 normal controls. Prior measurements of CSF total (t)\u2010\u03b1\u2010syn and the pS129\u2010\u03b1\u2010syn\/t\u2010\u03b1\u2010syn ratio were included for comparison.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The lower limit of quantification of the in\u2010house assay used to analyze CSF samples from participants was 0.57\u2009pg\/mL, which was well below the observed concentrations of endogenous pS129\u2010\u03b1\u2010syn in CSF. Median CSF pS129\u2010\u03b1\u2010syn levels were slightly lower in PD patients compared to controls (5.7\u2009pg\/mL vs. 6.5\u2009pg\/mL), but the difference was not significant in the unadjusted (<jats:italic>p<\/jats:italic>\u2009=\u20090.404) or adjusted analyses (<jats:italic>p<\/jats:italic>\u2009=\u20090.270). There was no difference in the pS129\u2010\u03b1\u2010syn\/t\u2010\u03b1\u2010syn ratio between patients and controls. Using linear mixed\u2010effects models, we found no association between baseline pS129\u2010\u03b1\u2010syn or the pS129\u2010\u03b1\u2010syn\/t\u2010\u03b1\u2010syn ratio and motor or cognitive decline over a 10\u2010year period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We developed and applied an in\u2010house SIMOA that reliably quantifies pS129\u2010\u03b1\u2010syn in CSF samples. This study does not indicate a role for CSF pS129\u2010\u03b1\u2010syn or the pS129\u2010\u03b1\u2010syn\/t\u2010\u03b1\u2010syn ratio as clinically useful diagnostic or prognostic biomarkers in PD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ene.70167","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T12:46:43Z","timestamp":1748868403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cerebrospinal Fluid Phosphorylated Alpha\u2010Synuclein in Newly Diagnosed Parkinson's Disease"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6521-741X","authenticated-orcid":false,"given":"Camilla Christina","family":"Pedersen","sequence":"first","affiliation":[{"name":"Centre for Movement Disorders Stavanger University Hospital  Stavanger Norway"},{"name":"Department of Chemistry, Bioscience and Environmental Engineering University of Stavanger  Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0630-2870","authenticated-orcid":false,"given":"Guido","family":"Alves","sequence":"additional","affiliation":[{"name":"Centre for Movement Disorders Stavanger University Hospital  Stavanger Norway"},{"name":"Department of Chemistry, Bioscience and Environmental Engineering University of Stavanger  Stavanger Norway"},{"name":"Department of Neurology Stavanger University Hospital  Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7230-6426","authenticated-orcid":false,"given":"Ole\u2010Bj\u00f8rn","family":"Tysnes","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine University of Bergen  Bergen Norway"},{"name":"Department of Neurology Haukeland University Hospital  Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7142-0078","authenticated-orcid":false,"given":"Jodi","family":"Maple\u2010Gr\u00f8dem","sequence":"additional","affiliation":[{"name":"Centre for Movement Disorders Stavanger University Hospital  Stavanger Norway"},{"name":"Department of Chemistry, Bioscience and Environmental Engineering University of Stavanger  Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0604-4891","authenticated-orcid":false,"given":"Johannes","family":"Lange","sequence":"additional","affiliation":[{"name":"Centre for Movement Disorders Stavanger University Hospital  Stavanger Norway"},{"name":"Department of Chemistry, Bioscience and Environmental Engineering University of Stavanger  Stavanger Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awad150"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/42166"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.56.1.33"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600933200"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb748"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2024.107240"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.27897"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010016\u20100072\u20109"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002566"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.28738"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40035\u2010020\u201000192\u20104"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.111"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2011.08.003"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/17576180.2024.2407718"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mds.28611"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1641"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvab251"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/ene.14032"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2008.168211"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.55.3.181"},{"key":"e_1_2_10_22_1","first-page":"153","article-title":"Unified Parkinson's Disease Rating Scale","volume":"2","author":"Fahn S.","year":"1987","journal-title":"Recent Developments in Parkinsons Disease"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.17.5.427"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20103956(75)90026\u20106"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2009.199950"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm\u20102016\u20100409"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000365637"},{"key":"e_1_2_10_28_1","unstructured":"\u201cR2_NAKAGAWA: Stata Module for Computing Nakagawa's R\u2010Squared Statistic for Multilevel Mixed\u2010Effects Linear Regression. Version S459345.\u201dBoston College Department of Economics.https:\/\/ideas.repec.org\/c\/boc\/bocode\/s459345.html2024."},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci11010119"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.51676"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2018.05.027"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/2024.02.17.24302981"}],"container-title":["European Journal of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ene.70167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T13:48:41Z","timestamp":1750945721000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ene.70167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1111\/ene.70167"],"URL":"https:\/\/doi.org\/10.1111\/ene.70167","archive":["Portico"],"relation":{},"ISSN":["1351-5101","1468-1331"],"issn-type":[{"value":"1351-5101","type":"print"},{"value":"1468-1331","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"2024-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70167"}}