{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T21:27:27Z","timestamp":1785446847936,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Euro J of Neurology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Skin biopsies and seed amplification assays (SAA) provide a sensitive and potentially quantitative method to detect alpha\u2010synuclein (a\u2010syn) aggregation in peripheral nerve fibers in Parkinson's disease (PD). Relating to the previously published hypothesis that PD may either originate in the peripheral (body\u2010first) or central (brain\u2010first) nervous system, we investigated whether patients with clinical features that have been reported to be associated with a suspected body\u2010first subtype of PD exhibit higher levels of a\u2010syn aggregation in dermal nerve fibers compared to those without these features. Patients with isolated REM sleep behavior disorder (iRBD) representing a suspected premotor stage of body\u2010first PD were studied in comparison to the PD cohort.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Patients were categorized on the basis of clinical features, and SAA parameters such as lag time, number of positive curves, and titers were analyzed and correlated with clinical features.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Although patients with clinical features of suspected body\u2010first PD showed slightly higher titers, significant differences were mainly observed between iRBD patients and PD patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data suggest that widespread \u03b1\u2010syn aggregation in advanced PD limits the use of SAA in skin biopsies for subtype differentiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ene.70453","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T06:36:04Z","timestamp":1764398164000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dermal Alpha\u2010Synuclein Aggregation in Seed Amplification Assays for Parkinson's Disease Subtype Differentiation"],"prefix":"10.1111","volume":"32","author":[{"given":"Magdalena","family":"Vieregge","sequence":"first","affiliation":[{"name":"Department of Neurology University Hospital W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Kuzkina","sequence":"additional","affiliation":[{"name":"Department of Neurology University Hospital W\u00fcrzburg  W\u00fcrzburg Germany"},{"name":"Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Janzen","sequence":"additional","affiliation":[{"name":"Department of Neurology Philipps University Marburg  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang H.","family":"Oertel","sequence":"additional","affiliation":[{"name":"Department of Neurology Philipps University Marburg  Marburg Germany"},{"name":"Helmholtz Center for Health and Environment, Institute for Neurogenomics  M\u00fcnchen\u2010Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Sommerauer","sequence":"additional","affiliation":[{"name":"Cognitive Neuroscience, Institute for Neuroscience and Medicine (INM\u20103) Research Centre Juelich  Juelich Germany"},{"name":"Department of Neurology, Faculty of Medicine and University Hospital Cologne, University of Cologne  Cologne Germany"},{"name":"German Centre for Neurodegenerative Diseases (DZNE), Bonn  Germany"},{"name":"Center of Neurology, Department of Parkinson, Sleep and Movement Disorders University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Volkmann","sequence":"additional","affiliation":[{"name":"Department of Neurology University Hospital W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2883-0009","authenticated-orcid":false,"given":"Kathrin","family":"Doppler","sequence":"additional","affiliation":[{"name":"Department of Neurology University Hospital W\u00fcrzburg  W\u00fcrzburg 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