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The time\u2010course of expression of this gene paralleled those of other genes encoding proteins involved during the secretory phase of odontogenesis, as described previously. Immunohistochemical studies of clusterin in murine molar tooth germs suggested this protein to be located in outer enamel epithelium, regressing enamel organ, secretory ameloblasts, and the dental epithelium connecting the tooth to the oral epithelium at an early eruptive stage. Immunolabelling of transforming growth factor beta\u20101 (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21) revealed it to be located close to clusterin. The levels of expression of <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lu<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>gfb1<\/jats:italic> were markedly decreased following in\u2010vivo transfection with anti\u2010mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u2010214. In contrast, the expression of several genes associated with regulation of growth and development were increased by this treatment. We suggest that clusterin has functions during secretory odontogenesis and the early eruptive phase. Bioinformatic analysis after treatment with anti\u2010mi<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\u2010214 suggested that, whilst cellular activities associated with tooth mineralization and eruption were inhibited, activities associated with an alternative developmental activity (i.e. biosynthesis of contractile proteins) appeared to be stimulated. These changes probably occur through regulation mediated by a common cluster of transcription factors and support suggestions that micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are highly significant as regulators of differentiation during odontogenesis.<\/jats:p>","DOI":"10.1111\/eos.12056","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T06:56:52Z","timestamp":1369724212000},"page":"303-312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Expression of <scp><i>Clu<\/i><\/scp> and <scp><i>Tgfb1<\/i><\/scp> during murine tooth development: effects of in\u2010vivo transfection with anti\u2010mi<scp>R<\/scp>\u2010214"],"prefix":"10.1111","volume":"121","author":[{"given":"Qalb\u2010E\u2010Saleem","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Oral 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