{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:22:01Z","timestamp":1773285721896,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,9,25]],"date-time":"2013-09-25T00:00:00Z","timestamp":1380067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:p>Dentin sialophosphoprotein (<jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content>) plays a vital role in dentinogenesis. Previously, we showed that, in addition to dentin, <jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> is also highly expressed in alveolar bone and cellular cementum, and plays a crucial role in maintaining periodontal integrity; <jats:italic><jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>spp<\/jats:italic>\u2010deficient mice demonstrate severe periodontal defects, including alveolar bone loss, decreased cementum deposition, abnormal osteocyte morphology in the alveolar bone, and apical migration of periodontal ligament. Dentin sialophosphoprotein in dentin and bone is cleaved into NH<jats:sub>2<\/jats:sub>\u2010terminal and <jats:styled-content style=\"fixed-case\">COOH<\/jats:styled-content>\u2010terminal fragments. Whilst our previous study showed that the proteolytic processing of <jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> is critical for dentinogenesis, it is unclear whether the post\u2010translational cleavage of <jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> also plays an essential role in maintaining a healthy periodontium. In this study, we analyzed the periodontal tissues from transgenic mice expressing the uncleavable full\u2010length <jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> in the <jats:italic>Dspp<\/jats:italic> knockout (<jats:italic>Dspp<\/jats:italic>\u2010KO) background (named \u2018<jats:italic>Dspp<\/jats:italic>\u2010KO\/D452A\u2010Tg mice\u2019), in comparison with those from wild\u2010type mice, <jats:italic>Dspp<\/jats:italic>\u2010KO mice, and mice expressing the normal <jats:italic>Dspp<\/jats:italic> transgene in the <jats:italic>Dspp<\/jats:italic>\u2010KO background (designated \u2018<jats:italic>Dspp<\/jats:italic>\u2010KO\/normal\u2010Tg mice\u2019). We found that transgenic expression of the normal DSPP fully rescued the periodontal defects of the <jats:italic>Dspp<\/jats:italic>\u2010KO mice, whereas this was not the case in <jats:italic>Dspp<\/jats:italic>\u2010KO\/D452A\u2010Tg mice. These results indicate that proteolytic processing of DSPP is essential to periodontal integrity.<\/jats:p>","DOI":"10.1111\/eos.12088","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T00:31:47Z","timestamp":1381537907000},"page":"545-550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Failure to process dentin sialophosphoprotein into fragments leads to periodontal defects in mice"],"prefix":"10.1111","volume":"121","author":[{"given":"Monica P.","family":"Gibson","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Center for Craniofacial Research and Diagnosis Texas A&amp;M University Baylor College of Dentistry Dallas TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyam","family":"Jani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Center for Craniofacial Research and Diagnosis Texas 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