{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T02:34:47Z","timestamp":1777602887746,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,1,24]],"date-time":"2014-01-24T00:00:00Z","timestamp":1390521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2014,4]]},"abstract":"Non\u2010syndromic cleft lip\/palate (NSCL<\/jats:styled-content>\/P<\/jats:styled-content>) is a common congenital defect inM<\/jats:styled-content>exico. Periconceptional intake of folic acid (FA<\/jats:styled-content>) may reduce the risk of this malformation. Although the 5,10\u2010methylenetetrahydrofolate reductase (MTHFR<\/jats:styled-content>) enzyme participates in folate metabolism, several studies failed to find any association betweenNSCL<\/jats:styled-content>\/P<\/jats:styled-content>and theMTHFR<\/jats:styled-content><\/jats:italic>C<\/jats:styled-content>677T<\/jats:styled-content>andA<\/jats:styled-content>1298C<\/jats:styled-content>polymorphisms. However, interactions amongNSCL<\/jats:styled-content>\/P<\/jats:styled-content>,MTHFR<\/jats:styled-content><\/jats:italic>gene polymorphisms, andFA<\/jats:styled-content>intake have not been explored inM<\/jats:styled-content>exican populations. This case\u2013control study included 132 patients withNSCL<\/jats:styled-content>\/P<\/jats:styled-content>and 370 controls fromM<\/jats:styled-content>exicoC<\/jats:styled-content>ity. MaternalFA<\/jats:styled-content>consumption during pregnancy was examined, as were theMTHFR<\/jats:styled-content><\/jats:italic>C<\/jats:styled-content>677T<\/jats:styled-content>andA<\/jats:styled-content>1298C<\/jats:styled-content>polymorphisms and gene\u2013FA<\/jats:styled-content>interactions. MaternalFA<\/jats:styled-content>intake during the periconceptional period was lower in cases (1.5%) than in controls (13%), with the risk of delivering a child withNSCL<\/jats:styled-content>\/P<\/jats:styled-content>lower in mothers who consumedFA<\/jats:styled-content>(OR<\/jats:styled-content>\u00a0=\u00a00.29, 95%CI<\/jats:styled-content>: 0.19\u20130.44). In addition, the risk ofNSCL<\/jats:styled-content>\/P was lower in children with theTT<\/jats:styled-content>than theCC<\/jats:styled-content>genotype ofMTHFR<\/jats:styled-content><\/jats:italic>C677T (OR<\/jats:styled-content>\u00a0=\u00a00.39, 95%CI<\/jats:styled-content>: 0.23\u20130.68), afterB<\/jats:styled-content>onferroni correction and exclusion of stratification. No evidence of gene\u2013FA<\/jats:styled-content>interaction was found. These results indicate that maternalFA<\/jats:styled-content>intake and theTT<\/jats:styled-content>genotype of theMTHFR<\/jats:styled-content><\/jats:italic>C<\/jats:styled-content>677T<\/jats:styled-content>polymorphism in children independently reduced the risk ofNSCL<\/jats:styled-content>\/P<\/jats:styled-content>in our population.<\/jats:p>","DOI":"10.1111\/eos.12114","type":"journal-article","created":{"date-parts":[[2014,1,24]],"date-time":"2014-01-24T14:36:13Z","timestamp":1390574173000},"page":"109-113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["5,10\u2010Methylenetetrahydrofolate reductase single nucleotide polymorphisms and gene\u2013environment interaction analysis in non\u2010syndromic cleft lip\/palate"],"prefix":"10.1111","volume":"122","author":[{"given":"Bernardette","family":"Estandia\u2010Ortega","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular Departamento de Gen\u00e9tica Humana Instituto Nacional de Pediatr\u00eda Distrito Federal Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A.","family":"Vel\u00e1zquez\u2010Arag\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular Departamento de Gen\u00e9tica Humana Instituto Nacional de Pediatr\u00eda Distrito Federal Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel A.","family":"Alc\u00e1ntara\u2010Ortigoza","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular Departamento de Gen\u00e9tica Humana Instituto Nacional de Pediatr\u00eda Distrito Federal Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam E.","family":"Reyna\u2010Fabian","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular Departamento de Gen\u00e9tica Humana Instituto Nacional de Pediatr\u00eda Distrito Federal Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Villag\u00f3mez\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Unidad de Apoyo a la Investigaci\u00f3n Cl\u00ednica Instituto Nacional de Pediatr\u00eda Distrito Federal Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariadna","family":"Gonz\u00e1lez\u2010del Angel","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular Departamento de Gen\u00e9tica Humana Instituto Nacional de Pediatr\u00eda Distrito Federal Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001665-200307000-00027"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1002\/bdra.20646","article-title":"Maternal fever during early pregnancy and the risk of oral clefts","volume":"88","author":"Hashmi S","year":"2010","journal-title":"Birth Defects Res A Clin Mol Teratol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200011303432204"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1597\/05-208.1"},{"key":"e_1_2_7_6_1","first-page":"213","article-title":"Tobacco smoking and oral clefts: a meta\u2010analysis","volume":"82","author":"Little J","year":"2004","journal-title":"Bull World Health Organ"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwm146"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20461"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20315"},{"key":"e_1_2_7_10_1","first-page":"1298","article-title":"Naturally ocurring forms of folic acid. 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