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In this study, we evaluated the effect of the S\u2010ribosylhomocysteine lyase (<jats:italic>luxS<\/jats:italic>) quorum\u2010sensing gene on <jats:italic>E. faecalis<\/jats:italic> biofilm formation by constructing a knockout mutant. The biofilms formed by both <jats:italic>E. faecalis<\/jats:italic> and its <jats:italic>luxS<\/jats:italic> mutant strain were evaluated using the <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> method. Important parameters that influence biofilm formation, including cell\u2010surface hydrophobicity and the nutrient content of the growth medium, were also studied. Biofilm structures were observed using confocal laser scanning microscopy (<jats:styled-content style=\"fixed-case\">CLSM<\/jats:styled-content>), and expression of biofilm\u2010related genes was investigated using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The results showed that the <jats:italic>luxS<\/jats:italic> gene can affect biofilm formation, whereas it does not affect the bacterial growth rate. Deletion of the <jats:italic>luxS<\/jats:italic> gene also increased cell\u2010surface hydrophobicity. Biofilm formation was accelerated by the addition of increasing concentrations of glucose. The <jats:styled-content style=\"fixed-case\">CLSM<\/jats:styled-content> images revealed that the <jats:italic>luxS<\/jats:italic> mutant strain tends to aggregate into distinct clusters and relatively dense structures, whereas the wild\u2010type strain appears confluent and more evenly distributed. All genes examined were up\u2010regulated in the biofilms formed by the <jats:italic>luxS<\/jats:italic> mutant strain. The quorum\u2010sensing <jats:italic>luxS<\/jats:italic> gene can affect <jats:italic>E. faecalis<\/jats:italic> biofilm formation.<\/jats:p>","DOI":"10.1111\/eos.12269","type":"journal-article","created":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T10:49:07Z","timestamp":1460717347000},"page":"234-240","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Effect of the quorum\u2010sensing <i>luxS<\/i> gene on biofilm formation by <i>Enterococcus faecalis<\/i>"],"prefix":"10.1111","volume":"124","author":[{"given":"Zhiyan","family":"He","sequence":"first","affiliation":[{"name":"Department of Endodontics Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai Research Institute of Stomatology Shanghai Key Laboratory of Stomatology Shanghai 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