{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T13:56:07Z","timestamp":1782827767420,"version":"3.54.5"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,14]],"date-time":"2016-06-14T00:00:00Z","timestamp":1465862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation is an important epigenetic mechanism involved in the regulation of gene expression, and a reduction in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation influences cell\u2010cycle progression and cell differentiation in inflammatory cells. The aim of the present study was to analyze the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010methylation pattern at local and global\/systemic levels in patients with periodontitis and gingivitis. Twenty\u2010one subjects with generalized, severe periodontitis and 17 subjects with gingival inflammation but no attachment loss were recruited. Gingival biopsies and peripheral blood samples were collected and prepared for immunohistochemical analysis of 5\u2010methylcytosine (5mC), 5\u2010hydroxymethylcytosine (5hmC), ten\u2010eleven translocation 2 (<jats:styled-content style=\"fixed-case\">TET<\/jats:styled-content>2), and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methyltransferase 1 (<jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>1). Whilst a similar pattern for 5mC and 5hmC <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation was found in both types of lesions, a significantly larger proportion of <jats:styled-content style=\"fixed-case\">TET<\/jats:styled-content>2\u2010positive cells was found in periodontitis lesions than in gingivitis lesions. Quantitative real\u2010time PCR analysis showed no differences between gingivitis and periodontitis lesions regarding expression of <jats:italic><jats:styled-content style=\"fixed-case\">TET<\/jats:styled-content>2<\/jats:italic> and isocitrate dehydrogenase (<jats:italic><jats:styled-content style=\"fixed-case\">IDH<\/jats:styled-content><\/jats:italic>) genes, while the global level of 5hmC was significantly higher in blood than in tissue in patients with periodontitis. It is suggested that epigenetic changes are more common in periodontitis lesions than in gingivitis lesions and that such changes are tissue specific.<\/jats:p>","DOI":"10.1111\/eos.12281","type":"journal-article","created":{"date-parts":[[2016,6,14]],"date-time":"2016-06-14T07:25:04Z","timestamp":1465889104000},"page":"329-333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Expression of TET2 enzyme indicates enhanced epigenetic modification of cells in periodontitis"],"prefix":"10.1111","volume":"124","author":[{"given":"Lena","family":"Larsson","sequence":"first","affiliation":[{"name":"Department of Periodontology The Sahlgrenska Academy at University of Gothenburg G\u00f6teborg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Thorbert\u2010Mros","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Sahlgrenska Academy at University of Gothenburg G\u00f6teborg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Lopez\u2010Lago","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Sahlgrenska Academy at University of Gothenburg G\u00f6teborg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Kalm","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Sahlgrenska Academy at University of Gothenburg G\u00f6teborg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asal","family":"Shikhan","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Sahlgrenska Academy at University of Gothenburg G\u00f6teborg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tord","family":"Berglundh","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Sahlgrenska Academy at University of Gothenburg G\u00f6teborg 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