{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T11:26:56Z","timestamp":1778153216974,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T00:00:00Z","timestamp":1484265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education, Science and Culture, Japan","award":["24592543"],"award-info":[{"award-number":["24592543"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>The aim of this study was to elucidate the relationship between the gustatory function and average number of taste buds per fungiform papilla (<jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content>) in humans. Systemically healthy volunteers (<jats:italic>n<\/jats:italic>\u00a0=\u00a0211), pre\u2010operative patients with chronic otitis media (<jats:italic>n<\/jats:italic>\u00a0=\u00a079), and postoperative patients, with or without a chorda tympani nerve (<jats:styled-content style=\"fixed-case\">CTN<\/jats:styled-content>) severed during middle ear surgery (<jats:italic>n<\/jats:italic>\u00a0=\u00a063), were included. Confocal laser scanning microscopy was employed to observe fungiform taste buds because it allows many <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> to be observed non\u2010invasively in a short period of time. Taste buds in an average of 10 <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> in the midlateral region of the tongue were counted. In total, 3,849 <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> were observed in 353 subjects. The gustatory function was measured by electrogustometry (<jats:styled-content style=\"fixed-case\">EGM<\/jats:styled-content>). An inverse relationship was found between the gustatory function and average number of fungiform taste buds per papilla. The healthy volunteers showed a lower <jats:styled-content style=\"fixed-case\">EGM<\/jats:styled-content> threshold (better gustatory function) and had more taste buds than did the patients with otitis media, and the patients with otitis media showed a lower <jats:styled-content style=\"fixed-case\">EGM<\/jats:styled-content> threshold and had more taste buds than did postoperative patients, reflecting the severity of damage to the <jats:styled-content style=\"fixed-case\">CTN<\/jats:styled-content>. It was concluded that the confocal laser scanning microscope is a very useful tool for using to observe a large number of taste buds non\u2010invasively.<\/jats:p>","DOI":"10.1111\/eos.12329","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T19:13:31Z","timestamp":1484334811000},"page":"44-48","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Relationship between gustatory function and average number of taste buds per fungiform papilla measured by confocal laser scanning microscopy in humans"],"prefix":"10.1111","volume":"125","author":[{"given":"Takehisa","family":"Saito","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery Faculty of Medical Sciences University of Fukui Fukui Japan"},{"name":"Research and Education Program for Life Science University of Fukui Fukui 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