{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:31:05Z","timestamp":1790368265848,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T00:00:00Z","timestamp":1518134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005300","name":"Sveriges Tandl\u00e4karf\u00f6rbund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005300","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005390","name":"Alfred \u00d6sterlunds Stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005390","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003173","name":"Crafoordska Stiftelsen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003173","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005934","name":"Malm\u00f6 H\u00f6gskola","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005934","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004817","name":"Lund University Medical Faculty Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004817","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>The human host defense peptide, <jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037, is an important player in the first line of defense against invading microorganisms. <jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 and its precursor, <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18, have been detected in unstimulated whole saliva but no reports showing <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in isolated, parotid, and\/or submandibular\/sublingual saliva have been presented. Here, we measured the levels of <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in human parotid and submandibular\/sublingual saliva and investigated the expression of <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in parotid and submandibular gland tissue. Parotid and submandibular\/sublingual saliva was collected from healthy volunteers, and the levels of <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in saliva were analyzed by dot blot, <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, and western blotting. Cellular expression of <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in human parotid and submandibular glands was investigated by immunohistochemistry. Immunoreactivity for <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 was detected in both parotid and submandibular\/sublingual saliva of all individuals. The concentration of <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 was similar in parotid and submandibular\/sublingual saliva, and was determined by densitometric scanning of each dot and normalization to the total protein concentration of each sample, and by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Double immunohistochemistry revealed that intravascular neutrophils of both parotid and submandibular glands express <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037. For the first time, we demonstrate <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in isolated human parotid and submandibular\/sublingual saliva and expression of <jats:styled-content style=\"fixed-case\">hCAP<\/jats:styled-content>18\/<jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 in glandular intravascular neutrophils, indicating that neutrophils of the major salivary glands contribute to the <jats:styled-content style=\"fixed-case\">LL<\/jats:styled-content>\u201037 content of whole saliva.<\/jats:p>","DOI":"10.1111\/eos.12407","type":"journal-article","created":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T09:38:44Z","timestamp":1518169124000},"page":"93-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The host defense peptide LL\u201037 is detected in human parotid and submandibular\/sublingual saliva and expressed in glandular neutrophils"],"prefix":"10.1111","volume":"126","author":[{"given":"Daniel","family":"Svensson","sequence":"first","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Aidoukovitch","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"},{"name":"Folktandv\u00e5rden Sk\u00e5ne Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Anders","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"Agerberth","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology Department of Laboratory Medicine Karolinska Institute Karolinska University Hospital Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Andersson","sequence":"additional","affiliation":[{"name":"Regional Laboratories Region Sk\u00e5ne Department of Pathology Sk\u00e5ne University Hospital Lund Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Ekblad","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Ericson","sequence":"additional","affiliation":[{"name":"Department of Cariology Faculty of Odontology Malm\u00f6 University Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Nebel","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrikke","family":"Voss","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2337-8412","authenticated-orcid":false,"given":"Bengt\u2010Olof","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science Lund University Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.29"},{"key":"e_1_2_7_3_1","first-page":"179","article-title":"The role of cathelicidins in the innate host defenses of mammals","volume":"7","author":"Zanetti M","year":"2005","journal-title":"Curr Issues Mol Biol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.12.3951"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-6075com"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1123933"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-015-0884-z"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2449-z"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.9.2206"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/35106587"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/b912533g"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08302.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.4.1291-1297.1995"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335901"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00962.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516679973"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2011.00607.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208101210"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.9.3883-3888.2005"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2013.01.003"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2016.05.009"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(82)90192-3"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200302000-00003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1075110"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12506462"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1988.tb00805.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2001.tb02801.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.5.837"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050191"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11201-3"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Feos.12407","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.12407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T12:38:25Z","timestamp":1695645505000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eos.12407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,9]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/eos.12407"],"URL":"https:\/\/doi.org\/10.1111\/eos.12407","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,9]]},"assertion":[{"value":"2017-12-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}