{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:55:38Z","timestamp":1790398538498,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771055"],"award-info":[{"award-number":["81771055"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE022549"],"award-info":[{"award-number":["DE022549"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:p>The glycosaminoglycan (<jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content>) chains attached to the core proteins of proteoglycans exert multiple roles, such as enriching signal molecules and regulating the binding of ligands to the corresponding receptors. A newly identified kinase \u2013 family with sequence similarity 20 member B (<jats:styled-content style=\"fixed-case\">FAM<\/jats:styled-content>20B) \u2013 is essential for the formation of <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> chains. The <jats:styled-content style=\"fixed-case\">FAM<\/jats:styled-content>20B protein phosphorylates the initial xylose on the side chain of a serine residue in the protein. Although the <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> chains of proteoglycans are believed to be indispensable during craniofacial development, there are few reports on their exact functions in craniofacial organogenesis. In this study, by mating Wnt1\u2010cre mice with Fam20b\u2010floxed mice (Fam20bflox\/flox), we created Wnt1\u2010Cre;Fam20bflox\/flox mice in which Fam20b is ablated in the neural crest\u2010derived mesenchyme. The Wnt1\u2010Cre;Fam20bflox\/flox mice died immediately after birth because of complete cleft palates. In addition to cleft palate, Wnt1\u2010Cre;Fam20bflox\/flox mice also manifested tongue elevation, micrognathia, microcephaly, suture widening, and reduced mineralization in the calvaria, facial bones, and temporomandibular joint. These findings indicate that the proteoglycans formed through the catalysis of <jats:styled-content style=\"fixed-case\">FAM<\/jats:styled-content>20B are essential for the morphogenesis and mineralization of the craniofacial complex.<\/jats:p>","DOI":"10.1111\/eos.12563","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T08:48:43Z","timestamp":1534236523000},"page":"433-436","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Inactivation of <i>Fam20b<\/i> in the neural crest\u2010derived mesenchyme of mouse causes multiple craniofacial defects"],"prefix":"10.1111","volume":"126","author":[{"given":"Xuenan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Radiology The 2nd Hospital Affiliated to Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&amp;M University College of Dentistry Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiao","family":"Ran","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbo","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&amp;M University College of Dentistry Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&amp;M University College of Dentistry Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlin","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis Texas A&amp;M University College of Dentistry Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3584-0224","authenticated-orcid":false,"given":"Chao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology College of Stomatology Dalian Medical University Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,14]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.60.070191.002303"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004952"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.06.006"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.2.193"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155412464638"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.416909"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090474"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417993111"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985812453177"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002246"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12222"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29814"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1671"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2014.05.001"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2015.02.003"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.06.027"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.208082"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2013.12.007"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.10.004"}],"updated-by":[{"DOI":"10.1111\/eos.12597","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T00:00:00Z","timestamp":1541980800000}}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Feos.12563","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.12563","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T18:19:36Z","timestamp":1694801976000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eos.12563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,14]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/eos.12563"],"URL":"https:\/\/doi.org\/10.1111\/eos.12563","archive":["Portico"],"relation":{"correction":[{"id-type":"doi","id":"10.1111\/eos.12597","asserted-by":"object"}]},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,14]]},"assertion":[{"value":"2018-07-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}