{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T22:32:46Z","timestamp":1768689166738,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371150"],"award-info":[{"award-number":["81371150"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771077"],"award-info":[{"award-number":["81771077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771085"],"award-info":[{"award-number":["81771085"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:p>Bacterial metabolism during infection is related to bacterial persistence and virulence factors. <jats:italic>Porphyromonas gingivalis<\/jats:italic> is a key pathogen that contributes to chronic periodontitis. Our previous study showed that <jats:italic>pckA<\/jats:italic>, the gene encoding phosphoenolpyruvate carboxykinase, is a putative\u2010specific pathogenic gene of virulent strains of <jats:italic>P. gingivalis<\/jats:italic>. Here, a <jats:italic>pckA<\/jats:italic>\u2010deficient strain (\u0394<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>1676) was constructed in <jats:italic>P. gingivalis<\/jats:italic> W83. Virulence properties were compared between the mutant and wild\u2010type strains. Specifically, hemagglutination activity was determined by the ability to agglutinate sheep erythrocytes. Gingipain activity was detected using synthetic\u2010specific substrates. Gene expression levels were analyzed using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010q<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, and cell surface\u2010associated polysaccharides were examined by silver staining and electron microscopy. Inactivation of the <jats:italic>pckA<\/jats:italic> gene did not affect bacterial growth and lipopolysaccharide formation but led to a reduction in hemagglutination activity and downregulation in expression of the hemagglutination\u2010associated gene, <jats:italic>rfa<\/jats:italic>, when compared with the wild\u2010type strain. Additionally, the \u0394<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>1676 mutant exhibited an alteration in the distribution of gingipain activity. Increased gingipain activity was detected on the cell surface, but a decrease in its activity in the culture supernatant was shown. Taken together, our results suggest that the <jats:italic>pckA<\/jats:italic> gene plays a role in modulating the virulence of <jats:italic>P. gingivalis<\/jats:italic> W83.<\/jats:p>","DOI":"10.1111\/eos.12565","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T14:51:13Z","timestamp":1534949473000},"page":"359-366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>pckA<\/i>\u2010deficient <i>Porphyromonas gingivalis<\/i> W83 shows reduction in hemagglutination activity and alteration in the distribution of gingipain activity"],"prefix":"10.1111","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9606-0953","authenticated-orcid":false,"given":"Leng","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Periodontics West China Hospital of Stomatology Sichuan University Chengdu China"},{"name":"State Key Laboratory of Oral Diseases National Clinical 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengcheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Periodontics West China Hospital of Stomatology Sichuan University Chengdu China"},{"name":"State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Periodontics West China Hospital of Stomatology Sichuan University Chengdu China"},{"name":"State Key Laboratory of Oral Diseases National Clinical 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