{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:41:21Z","timestamp":1790044881003,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005760","name":"G\u00f6teborgs Universitet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005760","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007212","name":"V\u00e4stra G\u00f6talandsregionen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007212","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>We sought to investigate site\u2010specific expression of bone\u2010regulatory factors expressed by human dental follicles and to compare the stimulated expression of tumour necrosis factor (ligand) superfamily, member 11\/tumour necrosis factor receptor superfamily, member 11b (<jats:italic>RANKL\/OPG<\/jats:italic>) in human dental follicle cells (HDFCs) from different patients. Analysis of bone\u2010regulatory markers in follicles from 12 different study participants was performed using RT\u2010qPCR and immunofluorescence; apical and coronal segments from each dental follicle were processed independently. Four additional dental follicles were used for cell cultures; HDFCs were precultured in osteogenic medium to initiate differentiation and thereafter cultured with 10<jats:sup>\u22126<\/jats:sup>M forskolin (FSK) to activate the protein kinase cAMP (PKA\/cAMP) signalling pathway and induce<jats:italic>RANKL<\/jats:italic>\/<jats:italic>OPG<\/jats:italic>expression. We demonstrate that<jats:italic>RANKL<\/jats:italic>expression is significantly higher in the coronal part of follicles than in the apical part. High levels of collagen type 1 (<jats:italic>COL1<\/jats:italic>)<jats:italic>,<\/jats:italic>alkaline phosphatase (<jats:italic>ALP<\/jats:italic>) and Gap\u2010junction protein, alpha 1, 43 kDa (<jats:italic>CX43<\/jats:italic>) were expressed, whereas expression of Sp7 transcription factor (<jats:italic>OSX)<\/jats:italic>, bone morphogenetic protein 2 (<jats:italic>BMP2<\/jats:italic>)<jats:italic>,<\/jats:italic>colony\u2010stimulating factor 1 (CSF\u20101), chemokine (C\u2010C motif) ligand 2 (<jats:italic>MCP1<\/jats:italic>), and<jats:italic>OPG<\/jats:italic>was low in all samples. The immunofluorescence localization of<jats:italic>CSF\u20101<\/jats:italic>,<jats:italic>MCP1<\/jats:italic>, osteocalcin (<jats:italic>OCN<\/jats:italic>)<jats:italic>, RANKL,<\/jats:italic>and<jats:italic>BMP2<\/jats:italic>was not specific for either part of the follicles. In conclusion, a consistently high expression of<jats:italic>CX43<\/jats:italic>suggests that gap\u2010junction communication in HDFCs is essential for the eruption process. Furthermore, the induced expression of<jats:italic>RANKL<\/jats:italic>in HDFCs varies significantly between individuals and may relate to clinical variations in tooth eruption.<\/jats:p>","DOI":"10.1111\/eos.12568","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T03:21:43Z","timestamp":1537068103000},"page":"439-448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Study on site\u2010specific expression of bone formation and resorption factors in human dental follicles"],"prefix":"10.1111","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7273-499X","authenticated-orcid":false,"given":"Pamela","family":"Uribe","sequence":"first","affiliation":[{"name":"Department of Orthodontics Institute of Odontology Sahlgrenska Academy University of Gothenburg Gothenburg 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