{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T18:08:33Z","timestamp":1773857313176,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T00:00:00Z","timestamp":1546819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Harvesting, expanding, and re\u2010implanting osteogenic mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) avoids the donor\u2010site morbidity associated with autogenous grafting from bone marrow. Mesenchymal stem cells sourced from the palatal periosteum could be an alternative to isolation of such cells using bone marrow aspiration procedures. For safe use in human therapy, <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s should be expanded in culture medium that is free from animal or human\u2010derived serum. In this study we localized, quantified, and characterized <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s from palatal periosteum cultured in serum\u2010free, xeno\u2010free Essential 8 medium. A portion of the palatal periosteum tissues from three patients were dual\u2010immunostained with <jats:styled-content style=\"fixed-case\">MSC\u2010<\/jats:styled-content>specific markers (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>90, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>73). The remaining portions were expanded in culture, and the isolated <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were analyzed using flow cytometry and tri\u2010lineage differentiation. Palatal periosteum sections were found to contain <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105\u2010, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>90\u2010, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>73\u2010positive cells. The cultured cells were 73.0\u00a0\u00b1\u00a06.7% (mean\u00a0\u00b1\u00a0<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>) positive for all three <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\u2010specific markers and were without hematopoietic stem cell (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>) markers 0.5\u00a0\u00b1\u00a00.3% (mean\u00a0\u00b1\u00a0SD). Tri\u2010lineage differentiation analysis confirmed that palatal periosteum cells could become adipoblasts, chondroblasts, and osteoblasts. The results demonstrate that palatal\u2010derived <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s could be detected in\u00a0situ within small niches, and when expanded in serum\u2010free, xeno\u2010free medium represent a viable source of <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s for clinical use.<\/jats:p>","DOI":"10.1111\/eos.12603","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T01:03:39Z","timestamp":1546995819000},"page":"99-111","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Localization and characterization of human palatal periosteum stem cells in serum\u2010free, xeno\u2010free medium for clinical use"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5404-2869","authenticated-orcid":false,"given":"Noel Ye","family":"Naung","sequence":"first","affiliation":[{"name":"Faculty of 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