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It is frequently treated with topical corticosteroids (applied as gels, mouthwashes, or sprays); however, the mucosal exposure times of topical corticosteroids are short because of removal by the constant flow of saliva and mechanical forces. In this study we used cell monolayers, as well as oral mucosal equivalents (OMEs) containing activated T\u2010cells, to examine corticosteroid potency and delivery of clobetasol\u201017\u2010propionate from a novel electrospun mucoadhesive patch. The OMEs displayed tight junctions, desmosomes, hemidesmosomes, and an efficient permeability barrier. Following application of corticosteroids to cells cultured as monolayers, the degree of cytotoxicity measured correlated to the level of potency recognized for each corticosteroid; by contrast, OMEs were largely unaffected by corticosteroid treatment. Permeation of clobetasol\u201017\u2010propionate into and through the OMEs was time\u2010 and dose\u2010dependent, regardless of whether this corticosteroid was delivered in liquid form or from a mucoadhesive patch, and both liquid\u2010 and patch\u2010delivered clobetasol\u201017\u2010propionate significantly reduced the secretion of interleukin\u20102 by activated T\u2010cells. This study confirms that OMEs are more suitable models than cell monolayers for evaluating toxicity and drug delivery. After topical exposure, clobetasol\u201017\u2010propionate accumulated in OMEs at a higher level than betamethasone\u201017\u2010valerate and hydrocortisone\u201017\u2010valerate, and exerted its immunosuppressive actions following application via the patch delivery system, highlighting the efficacy of this mode of drug delivery to treat OLP.<\/jats:p>","DOI":"10.1111\/eos.12761","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T12:12:05Z","timestamp":1614600725000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Corticosteroid delivery using oral mucosa equivalents for the treatment of inflammatory mucosal diseases"],"prefix":"10.1111","volume":"129","author":[{"given":"Zulfahmi","family":"Said","sequence":"first","affiliation":[{"name":"School of Clinical Dentistry University of Sheffield Sheffield UK"},{"name":"Faculty of Dentistry Universiti Sains Islam Malaysia Kuala Lumpur 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