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After applying several filters to an Illumina array, we extracted 175 single nucleotide polymorphisms (SNPs) from 150 mothers of NSCL\/P cases and 150 control women. Association was evaluated using computed odds ratio (OR) with a 95% confidence interval (95% CI) in additive, recessive, and dominant models. After multiple comparison correction, only SNP rs4451422 (A&gt;C), located 237\u00a0bp downstream of the gene encoding the human folylpolyglutamate synthetase (<jats:italic>FPGS<\/jats:italic>), maintained a significant association with NSCL\/P in the offspring (OR 3.03; 95% CI 1.69\u20135.26). The variant rs4451422 is associated with a decrease in <jats:italic>FPGS<\/jats:italic> expression according to database annotation. Our results lead to a new hypothesis that a lower activity of FPGS enzyme reduces intracellular folate levels and increases the risk of an offspring having NSCL\/P.<\/jats:p>","DOI":"10.1111\/eos.12813","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T17:48:04Z","timestamp":1626889684000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Maternal genotypes of folate\/one\u2010carbon metabolism gene variants and nonsyndromic cleft lip with or without cleft palate risk in Chile"],"prefix":"10.1111","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0786-2220","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Inostroza","sequence":"first","affiliation":[{"name":"Department of Anatomy, School of Medicine, Faculty of Medicine Pontificia Universidad Cat\u00f3lica de Chile  Santiago 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