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The aim of the present study was to determine whether local administration of EPA to adult male Wistar rats could attenuate the excitability of the jaw\u2010opening reflex in vivo, including nociception. The jaw\u2010opening reflex evoked by electrical stimulation of the tongue was recorded by a digastric muscle electromyogram (dEMG) in pentobarbital\u2010anesthetized rats. The amplitude of the dEMG response was significantly increased in proportion to the electrical stimulation intensity (1\u00d7\u20135\u00d7 threshold). At 3\u00d7, local administration of EPA dose\u2010dependently inhibited the dEMG response, lasting 60\u00a0min, with maximum inhibition observed within approximately 10\u00a0min. The mean magnitude of dEMG signal inhibition by EPA was almost equal to that observed with a local anesthetic, 1% lidocaine, and with a half dose of lidocaine plus a half dose of EPA. These findings suggest that EPA attenuates the jaw\u2010opening reflex, possibly by blocking Nav channels of primary nerve terminals, and strongly support the idea that EPA is a potential therapeutic agent and complementary alternative medicine for the prevention of acute trigeminal nociception.<\/jats:p>","DOI":"10.1111\/eos.12917","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T13:57:32Z","timestamp":1675778252000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of local administration of eicosapentaenoic acid on the jaw\u2010opening reflex in rats"],"prefix":"10.1111","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3348-4155","authenticated-orcid":false,"given":"Hibiki","family":"Osaki","sequence":"first","affiliation":[{"name":"Laboratory of Food and Physiological Sciences Department of Life and Food Sciences School of Life and Environmental 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