{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T23:06:17Z","timestamp":1787094377910,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Periodontitis is an inflammatory disease of tooth\u2010supporting tissues caused by oral bacteria. Periodontal ligament loss and alveolar bone destruction occur in progressive periodontitis. Since gingival crevicular fluids (GCF) reflects the inflammatory environment of the periodontal pocket, it is a very important specimen for developing targets for periodontitis diagnosis. An antibody array was performed using GCF collected from healthy participants and patients with periodontitis to identify the proteolytic enzymes involved in periodontitis. Of 21 targets on the antibody array membrane, kallikrein 6 (KLK6), kallikrein 10 (KLK10), cathepsin A (CathA), and cathepsin D (CathD) showed higher levels in periodontitis GCF than in GCF from healthy participants. Lipopolysaccharide stimulation of <jats:italic>Porphyromonas gingivalis<\/jats:italic> (PG\u2010LPS) in immortalized gingival fibroblasts only increased CathD protein levels among the four targets. The substrate cleavage activity of CathD was increased in PG\u2010LPS\u2010treated immortalized gingival fibroblast extract. The PG\u2010LPS\u2010induced substrate cleavage effect was abolished by the CathD inhibitor pepstatin A. Osteoclast formation was promoted by treatment with conditioned media from PG\u2010LPS\u2010 treated immortalized gingival fibroblasts but inhibited by the CathD inhibitor pepstatin A. These results suggest that PG\u2010LPS affected the osteoclast formation process by increasing CathD expression in cells around the alveolar bone, thereby participating in periodontitis progression.<\/jats:p>","DOI":"10.1111\/eos.12923","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T04:14:44Z","timestamp":1676434484000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Role of cathepsin D induced by <i>Porphyromonas gingivalis<\/i> lipopolysaccharide in periodontitis"],"prefix":"10.1111","volume":"131","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2772-1487","authenticated-orcid":false,"given":"Hyun Woong","family":"Jeong","sequence":"first","affiliation":[{"name":"Department of Dental Hygiene, College of Health, Science Eulji University  Seongnam Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1242-9894","authenticated-orcid":false,"given":"Dong Sik","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Eulji University Hospital, Eulji University  Daejeon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3367-6229","authenticated-orcid":false,"given":"June Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Seoul Hana Dental Clinic  Seongnam Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7012-3434","authenticated-orcid":false,"given":"Young Sun","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Dental Hygiene, College of Health, Science Eulji University  Seongnam Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071227"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13046"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.54.1.413"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00158.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0040-1712718"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4110\/in.2018.18.e8"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2017.05.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890600800103"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9071679"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.36.12.881"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2019.12.006"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020695"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2015.04.008"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4786170"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4140"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005965927185"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom5043260"},{"key":"e_1_2_8_20_1","first-page":"1924","article-title":"Cathepsin D level in the human gingival tissues and serum of periodontitis subjects","volume":"5","author":"Pushparani DS","year":"2014","journal-title":"Int J Pharm Sci Res"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11762"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2019.12.001"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00162.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1998.tb00706.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1515\/bmc-2012-0054"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2011.10.002"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)01349-6"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2017.291"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.12.036"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.10.035"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(99)00226-0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00271276"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092073"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.4068\/cmj.2016.52.1.12"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.655787"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106831\u201014\u201089"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00433"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/1758835920927838"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.140642"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.12923","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/eos.12923","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.12923","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T08:40:00Z","timestamp":1694508000000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eos.12923"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1111\/eos.12923"],"URL":"https:\/\/doi.org\/10.1111\/eos.12923","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,14]]},"assertion":[{"value":"2022-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12923"}}