{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:22:18Z","timestamp":1753878138396,"version":"3.41.2"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T00:00:00Z","timestamp":1737763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The periodontal ligament (PDL) is a connective tissue, and PDL cells have a potential to differentiate into cementoblasts, osteoblasts, and gingival fibroblasts. This study investigated whether transcription factor c\u2010Myb could induce differentiation of PDL cells for periodontal regeneration. PDL cells were isolated from extracted teeth and cultured. c\u2010Myb was transfected to PDL cells using replication\u2010deficient adenoviral vector. Differentiation of the PDL cells was analyzed by immunoblot, alkaline phosphatase activity, Alizarin red stain, and immunofluorescence analysis. Cell viability on titanium surfaces was analyzed by crystal violet stain and 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assay. PDL cells cultured in osteogenic medium showed increased production of osteogenic and cementogenic molecules. Moreover, c\u2010Myb\u2010transfected cells showed increased production of dentinogenic molecules, in addition to the osteogenic and cementogenic molecules, even in normal culture condition. c\u2010Myb\u2010transfected cells also exhibited increased autophagy and type I collagen production under nutrient deprivation. When grown on a titanium surface, c\u2010Myb\u2010transfected cells showed increased production of osteogenesis\u2010, dentinogenesis\u2010, and cementogenesis\u2010related molecules and cell viability. Thus, these results suggest that c\u2010Myb might play an essential role during periodontal regeneration by improving the differentiation of PDL cells, and c\u2010Myb can be utilized for enhancing the attachment of PDL cells to dental implant surfaces.<\/jats:p>","DOI":"10.1111\/eos.13040","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T10:30:37Z","timestamp":1737801037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of c\u2010Myb overexpression on osteoblastic\u2010, odontoblastic\u2010, and cementoblastic differentiation of primary human periodontal ligament cells"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8081-4367","authenticated-orcid":false,"given":"Young\u2010Hee","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry Jeonbuk National University  Jeonju\u2010si South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4403-3827","authenticated-orcid":false,"given":"Ho\u2010Keun","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry Jeonbuk National University  Jeonju\u2010si South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0274-1857","authenticated-orcid":false,"given":"Paras Man","family":"Pradhan","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry Jeonbuk National University  Jeonju\u2010si South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5071-8016","authenticated-orcid":false,"given":"Tae\u2010Kun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry Jeonbuk National University  Jeonju\u2010si South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6144-6899","authenticated-orcid":false,"given":"Sungil","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry, Institute of Oral Bioscience, School of Dentistry Jeonbuk National University  Jeonju\u2010si South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00094.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1990.tb01041.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2019.0025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2019.103259"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1997.68.4.320"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08434.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201080275\u20104"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2439"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515622139"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2018.1513940"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513497753"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23496"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/ams.ams_58_19"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12570"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(02)00896-2"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.5.2509"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.05.018"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.13112"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm12010018"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0219181"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2012.01.016"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23499"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2012.01004.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2011.01287.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-016-0219-2"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1168866"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4910399"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35527"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710140200"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.12.013"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000110079"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22770"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2003.11.016"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.12216"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.148.3.934"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094-013-1009-1"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2006.00985.x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.02.031"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.02.140"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13081370"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7039"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018\u2010023\u201004250\u20101"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.709408"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2004.03.006"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0909-8836.1998.eos106510.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200390152296"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-3233-2_6"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80258-5"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80259-7"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80257-3"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2012.0527"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509342873"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.06.065"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.2.380"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.12.7.17008"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.13040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T03:25:03Z","timestamp":1745551503000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eos.13040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,25]]},"references-count":55,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/eos.13040"],"URL":"https:\/\/doi.org\/10.1111\/eos.13040","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"type":"print","value":"0909-8836"},{"type":"electronic","value":"1600-0722"}],"subject":[],"published":{"date-parts":[[2025,1,25]]},"assertion":[{"value":"2024-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13040"}}