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Sixty space maintainer bands and 60 stainless steel crowns were divided into sterilized (Amsco Century V\u2010120 Prevac steam sterilizer) and non\u2010sterilized groups. These samples were immersed in artificial saliva, placed in individual petri dishes, and then incubated at 37\u00b0C. The samples were analyzed for 42 days using a gas chromatography\u2010mass spectrometry (GC\u2010MS) machine to quantify the release of nickel ions. Nickel release was higher in the sterilized samples than in the non\u2010sterilized samples for both space maintainer bands and stainless steel crowns. This trend persisted throughout most days of the experiment, with a peak release observed on day 7. Autoclave sterilization had a significant impact on ion release, raising potential concerns regarding the material's biocompatibility. Dentists should be mindful of the increased nickel release observed from sterilized crowns and bands, as this information could influence treatment decisions, especially in children who are predisposed to allergies.<\/jats:p>","DOI":"10.1111\/eos.70013","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:08:37Z","timestamp":1745366917000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of autoclave sterilization on nickel ion release from stainless steel crowns and space maintainer bands"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1354-9807","authenticated-orcid":false,"given":"Noura","family":"Alessa","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry and Orthodontics College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4311-977X","authenticated-orcid":false,"given":"Habah","family":"Alhamdan","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry King Abdulaziz Medical City  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1855-3207","authenticated-orcid":false,"given":"Shahad","family":"Alrobaish","sequence":"additional","affiliation":[{"name":"College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0513-7120","authenticated-orcid":false,"given":"Nisrin","family":"AlQahtani","sequence":"additional","affiliation":[{"name":"College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7668-4314","authenticated-orcid":false,"given":"Norah","family":"Alarifi","sequence":"additional","affiliation":[{"name":"College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2189-3210","authenticated-orcid":false,"given":"Manal","family":"Almutairi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry and Orthodontics College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-1855","authenticated-orcid":false,"given":"Latifa","family":"Alhowaish","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry and Orthodontics College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7257-3649","authenticated-orcid":false,"given":"Majedah","family":"Al\u2010Homaidhi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry and Orthodontics College of Dentistry King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4176-5750","authenticated-orcid":false,"given":"Reuof","family":"Alessa","sequence":"additional","affiliation":[{"name":"Department of Orthodontics King Abdulaziz Medical City  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"2402","article-title":"Space maintainers in dentistry: past to present","volume":"7","author":"Setia V","year":"2013","journal-title":"Clin Diagn Res"},{"key":"e_1_2_8_3_1","first-page":"78","article-title":"Comparison of canine overlap in pediatric patients requiring stainless steel crown placement under general anesthesia before and after the procedure","volume":"16","author":"Ghadimi S","year":"2019","journal-title":"Front Dent"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijdr.IJDR_761_18"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD005512.pub3"},{"key":"e_1_2_8_6_1","first-page":"817","article-title":"A split mouth randomized controlled clinical trial to compare the clinical performance of primary posterior esthetic and stainless steel crown","volume":"13","author":"Singh D","year":"2022","journal-title":"J Pharm Negat Results"},{"key":"e_1_2_8_7_1","first-page":"21","article-title":"Space maintainer \u2013 a review","volume":"11","author":"Jitesh S","year":"2019","journal-title":"Drug Invent Today"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph182010867"},{"key":"e_1_2_8_9_1","unstructured":"GatesA JakubowskiJA ReginaAC.Nickel toxicology. 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