{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:54:39Z","timestamp":1781848479822,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Root canal infections, particularly those involving\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , continue to pose significant challenges in endodontic therapy due to both their inherent resistance to conventional agents. This study aimed to evaluate the antimicrobial efficacy of a newly synthesized piperazine\u2013chalcone hybrid compound, both individually and in various formulations with calcium hydroxide, against these clinically relevant pathogens. The antimicrobial activities were assessed using the disk diffusion method over 24 and 48\u00a0h, whereas in silico analyses were conducted to further investigate the compound's molecular interactions, pharmacokinetics, and toxicity potential. Results demonstrated that the piperazine\u2013chalcone hybrid exhibited strong antimicrobial activity against both pathogens, with inhibition zone diameters ranging from approximately 26\u201332\u00a0mm against\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    and 18\u201328\u00a0mm against\n                    <jats:italic>C. albicans<\/jats:italic>\n                    , exceeding those observed for calcium hydroxide and standard antimicrobial controls. Molecular docking analysis revealed favorable binding interactions of the hybrid compound with\n                    <jats:sc>d<\/jats:sc>\n                    \u2010alanine:\n                    <jats:sc>d<\/jats:sc>\n                    \u2010alanine ligase, with a docking score of \u22128.6\u00a0kcal\/mol. In silico toxicological assessment predicted a favorable safety profile, with an LD\n                    <jats:sub>50<\/jats:sub>\n                    value &gt;5000\u00a0mg\/kg and no major toxicity alerts. These findings suggest that the piperazine\u2013chalcone hybrid holds promise as a safe and effective candidate for enhanced endodontic disinfection.\n                  <\/jats:p>","DOI":"10.1111\/eos.70115","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:45:13Z","timestamp":1781847913000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative evaluation of a novel piperazine\u2013chalcone hybrid and calcium hydroxide formulations against root canal pathogens"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-4774","authenticated-orcid":false,"given":"Cenk Serhan","family":"Ozverel","sequence":"first","affiliation":[{"name":"DESAM Research Institute Near East University  Nicosia T\u00fcrkiye"},{"name":"Basic Medical Sciences Department Faculty of Dentistry Near East University Nicosia T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1431-935X","authenticated-orcid":false,"given":"Emine","family":"Erdag","sequence":"additional","affiliation":[{"name":"Basic Medical Sciences Department Faculty of Dentistry Near East University Nicosia T\u00fcrkiye"},{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Near East University Nicosia T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0721-7547","authenticated-orcid":false,"given":"Halil Doruk","family":"Kaynarca","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology Faculty of Veterinary Medicine Near East University Nicosia T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2072-501X","authenticated-orcid":false,"given":"Ege","family":"Namsoy","sequence":"additional","affiliation":[{"name":"EgeDent Dental Clinic Kyrenia T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4515-0267","authenticated-orcid":false,"given":"Hasan Guney","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry Near East University Nicosia T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/froh.2021.672887"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2023.2184924"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.01.002"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1590\/S1678-77572008000600002","article-title":"Efficacy of sodium hypochlorite and chlorhexidine against Enterococcus faecalis: a systematic 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