{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:43:48Z","timestamp":1792669428486,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/02823\u20103"],"award-info":[{"award-number":["2022\/02823\u20103"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2023\/05652\u20108"],"award-info":[{"award-number":["2023\/05652\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to assess the cytotoxicity of a novel polymerization initiator,\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl (\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyldimethylsilyl) glyoxylate (DKSi), and its effect on cytokine release by human dental pulp stem cells (hDPSCs), compared with camphorquinone (CQ) and diphenyl(2,4,6\u2010trimethylbenzoyl)phosphine oxide (TPO). hDPSCs were isolated from healthy third molars, seeded in 96\u2010well plates, and exposed to initiators diluted in dimethyl sulfoxide (0.1\u20136\u00a0mM) for 24\u00a0h. Mitochondrial metabolism was assessed by the MTT assay, and cytokine release (interleukin\u20101 beta, interleukin\u20106 [IL\u20106], interleukin\u20108 [IL\u20108], interleukin\u201010, and tumor necrosis factor alpha [TNF\u2010\u03b1]) was analyzed using the MAGPIX system. Untreated cells served as controls, and data were analyzed using two\u2010way\n                    <jats:sc>anova<\/jats:sc>\n                    and Tukey's test. All compounds showed low toxicity at lower concentrations, whereas 6\u00a0mM caused the greatest reduction in mitochondrial metabolism. DKSi also exhibited significant toxicity at 4\u00a0mM. Unlike CQ and TPO, DKSi did not promote cytokine release. TPO upregulated all cytokines tested, and CQ increased TNF\u2010\u03b1, IL\u20106, and IL\u20108. DKSi showed significant cytotoxicity only at high concentrations and a more limited modulation of cytokine release than CQ and TPO. These findings provide an initial biological characterization of DKSi and support its further investigation as a potential photoinitiator for dental resin formulations.\n                  <\/jats:p>","DOI":"10.1111\/eos.70149","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:21:36Z","timestamp":1792668096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human dental pulp stem cell response to a novel photoinitiator for resin\u2010based dental materials"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6523-763X","authenticated-orcid":false,"given":"Giulia Gamero","family":"Pizzanelli","sequence":"first","affiliation":[{"name":"Dental Research Division Paulista University  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9971-0568","authenticated-orcid":false,"given":"Matheus","family":"Kury","sequence":"additional","affiliation":[{"name":"Dental Research Division Paulista University  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2442-4444","authenticated-orcid":false,"given":"Jean\u2010Pierre","family":"Salomon","sequence":"additional","affiliation":[{"name":"Medical Devices and Dental Biomaterials, Nancy Dental Surgery Faculty University of Lorraine  Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9297-0335","authenticated-orcid":false,"given":"Jacques","family":"Lalev\u00e9e","sequence":"additional","affiliation":[{"name":"Institut de Science des Mat\u00e9riaux de Mulhouse Universit\u00e9 de Haute\u2010Alsace  Mulhouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0049-0201","authenticated-orcid":false,"given":"Denise Carleto","family":"Andia","sequence":"additional","affiliation":[{"name":"Dental Research Division Paulista University  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1507-2264","authenticated-orcid":false,"given":"Adriano F.","family":"Lima","sequence":"additional","affiliation":[{"name":"Dental Research Division Paulista University  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jdent.2017.06.002","article-title":"Repair may increase survival of direct posterior restorations\u2014a practice based study","volume":"64","author":"Casagrande L","year":"2017","journal-title":"J Dent"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1016\/j.dental.2019.04.008","article-title":"Performance of ceramic laminate veneers with immediate dentine sealing: an 11 year prospective clinical trial","volume":"35","author":"Gresnigt MMM","year":"2019","journal-title":"Dent Mater"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"621","DOI":"10.3390\/polym17050621","article-title":"Comparative evaluation of shear bond strength of aesthetic orthodontic brackets bonded to aged composite restorative resin materials","volume":"17","author":"Sayed ME","year":"2025","journal-title":"Polymers (Basel)"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jmbbm.2012.01.018","article-title":"Mechanical properties and degree of conversion of etch\u2010and\u2010rinse and self\u2010etch adhesive systems cured by a quartz tungsten halogen lamp and a light\u2010emitting diode","volume":"12","author":"Gaglianone LA","year":"2012","journal-title":"J Mech Behav Biomed Mater"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.2341\/10-066-L","article-title":"Degree of conversion of etch\u2010and\u2010rinse and self\u2010etch adhesives light\u2010cured using QTH or LED","volume":"35","author":"Faria\u2010e\u2010Silva AL","year":"2010","journal-title":"Oper Dent"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmbbm.2020.103875","article-title":"Chemistry of novel and contemporary resin\u2010based dental adhesives","volume":"110","author":"Dressano D","year":"2020","journal-title":"J Mech Behav Biomed Mater"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.dental.2006.06.005","article-title":"Quantum yield of conversion of the photoinitiator camphorquinone","volume":"23","author":"Chen YC","year":"2007","journal-title":"Dent Mater"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.dental.2008.01.007","article-title":"Influence of photoinitiator type on the rate of polymerization, degree of conversion, hardness and yellowing of dental resin composites","volume":"24","author":"Schneider LF","year":"2008","journal-title":"Dent Mater"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"e29","DOI":"10.1016\/j.dental.2019.11.008","article-title":"Evaluation of alternative photoinitiator systems in two\u2010step self\u2010etch adhesive systems","volume":"36","author":"Almeida SM","year":"2020","journal-title":"Dent Mater"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1590\/0103-6440201700841","article-title":"Influence of photoinitiator system on physical\u2010chemical properties of experimental self\u2010adhesive composites","volume":"28","author":"Bertolo MV","year":"2017","journal-title":"Braz Dent J"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/j.jdent.2004.11.013","article-title":"Molar extinction coefficients and the photon absorption efficiency of dental photoinitiators and light curing units","volume":"33","author":"Neumann MG","year":"2005","journal-title":"J Dent"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jmbbm.2019.06.005","article-title":"Increased rates of photopolymerisation by ternary type II photoinitiator systems in dental resins","volume":"98","author":"Lima AF","year":"2019","journal-title":"J Mech Behav Biomed Mater"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"6911","DOI":"10.1021\/acs.macromol.7b01370","article-title":"Silyl glyoxylates as a new class of high performance photoinitiators: blue LED induced polymerization of methacrylates in thin and thick films","volume":"50","author":"Bouzrati\u2010Zerelli M","year":"2017","journal-title":"Macromolecules"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.3390\/ijms22031480","article-title":"Inflammatory response mechanisms of the dentine\u2010pulp complex and the periapical tissues","volume":"22","author":"Galler KM","year":"2021","journal-title":"Int J Mol Sci"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1002\/jbm.b.35218","article-title":"Co\u2010initiators of polymerization can modulate the inflammatory cytokine release without major cytotoxic effects in human dental pulp cells","volume":"111","author":"Agostinelli BG","year":"2023","journal-title":"J Biomed Mater Res B Appl Biomater"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"1692","DOI":"10.1016\/j.dental.2024.07.018","article-title":"Novel co\u2010initiators of polymerization: cytotoxicity profile and modulation of inflammatory mediators in human dental pulp stem cells","volume":"40","author":"Lima AF","year":"2024","journal-title":"Dent Mater"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1435054","article-title":"Cytokines in gingivitis and periodontitis: from pathogenesis to therapeutic targets","volume":"15","author":"Neurath N","year":"2024","journal-title":"Front Immunol"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0099-2399(97)80205-1","article-title":"Stimulation of interleukin\u20101 beta production of human dental pulp cells by porphyromonas endodontalis lipopolysaccharide","volume":"23","author":"Hosoya S","year":"1997","journal-title":"J Endod"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.dental.2024.03.004","article-title":"Toxicity and cytokine release from human dental pulp stem cells after exposure to universal dental adhesives cured by single peak and polywave LEDs","volume":"40","author":"Lima AF","year":"2024","journal-title":"Dent Mater"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1016\/j.dental.2024.09.014","article-title":"Inflammatory and adhesion profile of gingival fibroblasts to lithium disilicate ceramic surfaces","volume":"40","author":"Lima JFC","year":"2024","journal-title":"Dent Mater"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/j.dental.2024.05.021","article-title":"Evaluating antimicrobial, cytotoxic and immunomodulatory effects of glass ionomer cement modified by chitosan and hydroxyapatite","volume":"40","author":"Colonello GP","year":"2024","journal-title":"Dent Mater"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.dental.2024.03.004","article-title":"Toxicity and cytokine release from human dental pulp stem cells after exposure to universal dental adhesives cured by single peak and polywave LEDs","volume":"40","author":"Lima AF","year":"2024","journal-title":"Dent Mater"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1097\/00004770-200104000-00008","article-title":"Regulation of interleukin\u20106 expression in human dental pulp cell cultures stimulated with Prevotella intermedia lipopolysaccharide","volume":"27","author":"Tokuda M","year":"2001","journal-title":"J Endod"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"2750","DOI":"10.3390\/ma12172750","article-title":"Solvent and HEMA increase adhesive toxicity and cytokine release from dental pulp cells","volume":"12","author":"Massaro H","year":"2019","journal-title":"Materials (Basel)"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.joen.2012.09.006","article-title":"Camphorquinone inhibits odontogenic differentiation of dental pulp cells and triggers release of inflammatory cytokines","volume":"39","author":"Kim RH","year":"2013","journal-title":"J Endod"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"9425","DOI":"10.3390\/ijms23169425","article-title":"Potentiality and inflammatory marker expression are maintained in dental pulp cell cultures from carious teeth","volume":"23","author":"Arora S","year":"2022","journal-title":"Int J Mol Sci"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.cyto.2022.155846","article-title":"The detection of pro\u2010inflammatory cytokines in exudates from dental pulp tissues","volume":"153","author":"Wu Q","year":"2022","journal-title":"Cytokine"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1097\/00004770-200301000-00013","article-title":"Interleukin\u201010 receptor expression in human dental pulp cells in response to lipopolysaccharide from Prevotella intermedia","volume":"29","author":"Tokuda M","year":"2003","journal-title":"J Endod"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1097\/00004770-200203000-00008","article-title":"Interleukin\u201010 inhibits expression of interleukin\u20106 and \u20108 mRNA in human dental pulp cell cultures via nuclear factor\u2010kappaB deactivation","volume":"28","author":"Tokuda M","year":"2002","journal-title":"J Endod"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.immuni.2008.03.003","article-title":"Contextual regulation of inflammation: a duet by transforming growth factor\u2010beta and interleukin\u201010","volume":"28","author":"Li MO","year":"2008","journal-title":"Immunity"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.phrs.2008.05.013","article-title":"Inflammatory and immunological aspects of dental pulp repair","volume":"58","author":"Goldberg M","year":"2008","journal-title":"Pharmacol Res"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.70149","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/eos.70149","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eos.70149","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:21:40Z","timestamp":1792668100000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eos.70149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,22]]},"references-count":31,"alternative-id":["10.1111\/eos.70149"],"URL":"https:\/\/doi.org\/10.1111\/eos.70149","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,22]]},"assertion":[{"value":"2026-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70149"}}