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However, mechanistic understanding of FCDII fails to keep pace with genetic discoveries, primarily due to the significant challenge in developing a clinically relevant animal model. Conceptually and clinically important questions, such as the unknown latent period of epileptogenesis and the controversial epileptogenic zone, remain unknown in all experimental FCDII animal models, making it even more challenging to investigate the underlying epileptogenic mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we used continuous video\u2010electroencephalography (EEG) monitoring to detect the earliest interictal and ictal events in a clustered regularly interspaced short palindromic repeats (CRISPR)\u2010in utero electroporation (IUE) FCDII rat model that shares genetic, pathological, and electroclinical signatures with those observed in humans. We then took advantage of in vivo local field potential (LFP) recordings to localize the epileptogenic zone in these animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>To the best of our knowledge, we showed for the first time that epileptiform discharges emerged during the third postnatal week, and that the first seizure occurred as early as during the fourth postnatal week. We also showed that both interictal and ictal discharges are localized within the dysplastic cortex, concordant with human clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>Together, our work identified the temporal and spatial frame of epileptogenesis in a highly clinically relevant FCDII animal model, paving the way for mechanistic studies at molecular, cellular, and circuitry levels.<\/jats:p><\/jats:sec>","DOI":"10.1111\/epi.16868","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T18:27:06Z","timestamp":1616092026000},"page":"1268-1279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Defining the latent period of epileptogenesis and epileptogenic zone in a focal cortical dysplasia type II (FCDII) rat model"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7525-983X","authenticated-orcid":false,"given":"Hsin\u2010Yi","family":"Kao","sequence":"first","affiliation":[{"name":"Department of Neurology University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9179-7411","authenticated-orcid":false,"given":"Shuntong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan Ann Arbor USA"},{"name":"Department of Neurology the Third Xiangya Hospital Central South University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Temenuzhka","family":"Mihaylova","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Ziobro","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EunSeon","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Psychology University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carli","family":"Fine","sequence":"additional","affiliation":[{"name":"Department of Psychology University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Brang","sequence":"additional","affiliation":[{"name":"Department of Psychology University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendon O.","family":"Watson","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of Michigan Ann Arbor USA"},{"name":"Molecular and Behavioral Neuroscience Institute University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2489-1288","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Michigan Ann Arbor USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535759719888646"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.00250"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2015.03.034"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000000531"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25272"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1703784"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-052016-100138"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2599"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41436-018-0060-2"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-02052-6"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-02061-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21423"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a022368"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnp.2019.06.001"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnp.2017.07.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/epi4.12044"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4084"},{"key":"e_1_2_8_19_1","volume-title":"Rigid body registration. 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