{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:21:41Z","timestamp":1792516901934,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2020\u2010116403RB\u2010I00 funded by MCIN\/AEI\/10.13039\/5"],"award-info":[{"award-number":["PID2020\u2010116403RB\u2010I00 funded by MCIN\/AEI\/10.13039\/5"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Epilepsia"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Ischemic stroke is one of the main causes of death and disability worldwide and currently has limited treatment options. Electroencephalography (EEG) signals are significantly affected in stroke patients during the acute stage. In this study, we preclinically characterized the brain electrical rhythms and seizure activity during the hyperacute and late acute phases in a hemispheric stroke model with no reperfusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>EEG signals and seizures were studied in a model of hemispheric infarction induced by permanent occlusion of the middle cerebral artery (pMCAO), which mimics the clinical condition of stroke patients with permanent ischemia. Electrical brain activity was also examined using a photothrombotic (PT) stroke model. In the PT model, we induced a similar (PT group\u20101) or smaller (PT group\u20102) cortical lesion than in the pMCAO model. For all models, we used a nonconsanguineous mouse strain that mimics human diversity and genetic variation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The pMCAO hemispheric stroke model exhibited thalamic\u2010origin nonconvulsive seizures during the hyperacute stage that propagated to the thalamus and cortex. The seizures were also accompanied by progressive slowing of the EEG signal during the acute phase, with elevated delta\/theta, delta\/alpha, and delta\/beta ratios. Cortical seizures were also confirmed in the PT stroke model of similar lesions as in the pMCAO model, but not in the PT model of smaller injuries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>In the clinically relevant pMCAO model, poststroke seizures and EEG abnormalities were inferred from recordings of the contralateral hemisphere (noninfarcted hemisphere), emphasizing the reciprocity of interhemispheric connections and that injuries affecting one hemisphere had consequences for the other. Our results recapitulate many of the EEG signal hallmarks seen in stroke patients, thereby validating this specific mouse model for the examination of the mechanistic aspects of brain function and for the exploration of the reversion or suppression of EEG abnormalities in response to neuroprotective and anti\u2010epileptic therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/epi.17675","type":"journal-article","created":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T08:16:14Z","timestamp":1686125774000},"page":"2499-2514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Preclinical examination of early\u2010onset thalamic\u2010cortical seizures after hemispheric stroke"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4928-0213","authenticated-orcid":false,"given":"Pablo","family":"Garc\u00eda\u2010Pe\u00f1a","sequence":"first","affiliation":[{"name":"Center for Biomedical Technology (CTB) Universidad Polit\u00e9cnica de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5798-9508","authenticated-orcid":false,"given":"Milagros","family":"Ramos","sequence":"additional","affiliation":[{"name":"Center for Biomedical Technology (CTB) Universidad Polit\u00e9cnica de Madrid  Madrid Spain"},{"name":"Departamento de Tecnolog\u00eda Fot\u00f3nica y Bioingenier\u00eda, ETSI Telecomunicaciones Universidad Polit\u00e9cnica de Madrid  Madrid Spain"},{"name":"Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (CIBER\u2010BBN)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7847-8707","authenticated-orcid":false,"given":"Juan M.","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Instrumentation and Applied Acoustics Research Group (I2A2) Universidad Polit\u00e9cnica de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3657-3890","authenticated-orcid":false,"given":"Ricardo","family":"Martinez\u2010Murillo","sequence":"additional","affiliation":[{"name":"Department of Translational Neuroscience Instituto Cajal (CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1699-7389","authenticated-orcid":false,"given":"Guillermo","family":"de Arcas","sequence":"additional","affiliation":[{"name":"Instrumentation and Applied Acoustics Research Group (I2A2) Universidad Polit\u00e9cnica de Madrid  Madrid Spain"},{"name":"Departamento de Ingenier\u00eda Mec\u00e1nica, ETSI Industriales Universidad Polit\u00e9cnica de Madrid  Madrid Spain"},{"name":"Laboratorio de Neuroac\u00fastica Universidad Polit\u00e9cnica de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-729X","authenticated-orcid":false,"given":"Daniel","family":"Gonzalez\u2010Nieto","sequence":"additional","affiliation":[{"name":"Center for Biomedical Technology (CTB) Universidad Polit\u00e9cnica de Madrid  Madrid Spain"},{"name":"Departamento de Tecnolog\u00eda Fot\u00f3nica y Bioingenier\u00eda, ETSI Telecomunicaciones Universidad Polit\u00e9cnica de Madrid  Madrid Spain"},{"name":"Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (CIBER\u2010BBN)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.029606"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2012.07.003"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2006.06.718"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2007.07.021"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00978.2015"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/ANA.0000000000000074"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000122622.73916.d2"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.2018.1455014"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNP.0000000000000166"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2020.2264"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000130989.17100.96"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seizure.2017.03.009"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42466-021-00161-w"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(02)00013-4"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737175.2017.1369880"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2012.05.031"},{"issue":"2","key":"e_1_2_12_18_1","first-page":"167","article-title":"The effects of analgesic supplements on neural activity in the main olfactory bulb of the mouse","volume":"57","author":"Mast TG","year":"2007","journal-title":"Comp Med"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00266.2021"},{"key":"e_1_2_12_20_1","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1079\/9780851998206.0000","volume-title":"Biostatistics for animal science","author":"Kaps M","year":"2004"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3950"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afz163"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.25.11.2204"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000144649.49861.1d"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75379-w"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01199774"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161025605"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3233\/RNN-120244"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/00207454.2016.1189913"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0987-7053(98)80115-9"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0013-4694(81)90007-9"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.251331"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clineuro.2006.06.003"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1088\/0967-3334\/34\/4\/423"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/sleep\/29.10.1339"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2003.10.065"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23836"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.114.006974"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.60.3.400"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2007.01513.x"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11517-020-02280-z"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12873-022-00585-w"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yebeh.2019.106472"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.105999"},{"issue":"3","key":"e_1_2_12_45_1","first-page":"715","article-title":"Early seizures in patients with acute stroke: frequency, predictive factors, and effect on clinical outcome","volume":"4","author":"Alberti A","year":"2008","journal-title":"Vasc Health Risk Manag"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.28.8.1585"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101914108"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3269"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8112006"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-06307-3"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18793324"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5180-07.2008"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405461200"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.wnr.0000220136.98918.41"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.024156"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0314-8"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-018-1859-2"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2018.02.002"}],"container-title":["Epilepsia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/epi.17675","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T19:54:38Z","timestamp":1700682878000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/epi.17675"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,19]]},"references-count":57,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1111\/epi.17675"],"URL":"https:\/\/doi.org\/10.1111\/epi.17675","archive":["Portico"],"relation":{},"ISSN":["0013-9580","1528-1167"],"issn-type":[{"value":"0013-9580","type":"print"},{"value":"1528-1167","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,19]]},"assertion":[{"value":"2022-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}