{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T20:48:14Z","timestamp":1784666894898,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771476"],"award-info":[{"award-number":["81771476"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871078"],"award-info":[{"award-number":["81871078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071536"],"award-info":[{"award-number":["82071536"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271578"],"award-info":[{"award-number":["82271578"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017291","name":"Zhongshan Science and Technology Bureau","doi-asserted-by":"publisher","award":["CXTD2022013"],"award-info":[{"award-number":["CXTD2022013"]}],"id":[{"id":"10.13039\/501100017291","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Epilepsia"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Methyl CpG\u2010binding protein 2 (MECP2) duplication syndrome is a rare X\u2010linked genomic disorder affecting predominantly males, which is usually manifested as epilepsy and autism spectrum disorder (ASD) comorbidity. The transgenic line MeCP2<jats:sup>Tg1<\/jats:sup> was used for mimicking MECP2 duplication syndrome and showed autism\u2013epilepsy co\u2010occurrence. Previous works suggested that the excitatory\/inhibitory (E\/I) imbalance is a potential common mechanism for both epilepsy and ASD. The projection neurons and parvalbumin (PV) interneurons account for the majority of E\/I balance in the hippocampus. Therefore, we explored how structural changes of projection and PV<jats:sup>+<\/jats:sup> neurons occur in the hippocampus of MeCP2<jats:sup>Tg1<\/jats:sup> mice and whether these morphological changes contribute to epilepsy susceptibility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used the interneuron Designer receptors exclusively activated by designer drugs mouse model to inhibit inhibitory neurons in the hippocampus to verify the epilepsy susceptibility of MeCP2<jats:sup>Tg1<\/jats:sup> (FVB, an inbred strain named as sensitivity to Friend leukemia virus) mice. Electroencephalograms were recorded for the definition of seizure. We performed retro\u2010orbital injection of virus in MeCP2<jats:sup>Tg1<\/jats:sup> (FVB):CaMKII\u03b1\u2010Cre (C57BL\/6) mice or MeCP2<jats:sup>Tg1<\/jats:sup>:PV\u2010Cre (C57BL\/6) mice and their littermate controls to specifically label projection and PV<jats:sup>+<\/jats:sup> neurons for structural analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Epilepsy susceptibility was increased in MeCP2<jats:sup>Tg1<\/jats:sup> mice. There was a reduced number of PV neurons and reduced dendritic complexity in the hippocampus of MeCP2<jats:sup>Tg1<\/jats:sup> mice. The dendritic complexity in MeCP2<jats:sup>Tg1<\/jats:sup> mice was increased compared to wild\u2010type mice, and total dendritic spine density in dentate gyrus of MeCP2<jats:sup>Tg1<\/jats:sup> mice was also increased. Total dendritic spine density was increased in CA1 of MeCP2<jats:sup>Tg1<\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>Overexpression of MeCP2 may disrupt crucial signaling pathways, resulting in decreased dendritic complexity of PV interneurons and increased dendritic spine density of projection neurons. This reciprocal modulation of excitatory and inhibitory neuronal structures associated with MeCP2 implies its significance as a potential target in the development of epilepsy and offers a novel perspective on the co\u2010occurrence of autism and epilepsy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/epi.18027","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T14:55:06Z","timestamp":1717167306000},"page":"2483-2496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Imbalance between hippocampal projection cell and parvalbumin interneuron architecture increases epileptic susceptibility in mouse model of methyl <scp>CpG<\/scp> binding protein 2 duplication syndrome"],"prefix":"10.1111","volume":"65","author":[{"given":"Junye","family":"Ge","sequence":"first","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjun","family":"Xie","sequence":"additional","affiliation":[{"name":"Jingzhou Hospital affiliated with Yangtze University  Jingzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamei","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biqing","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erling","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghui","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Southern Medical University  Guangzhou China"},{"name":"Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica Chinese Academy of Sciences  Zhongshan China"},{"name":"Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8344-0102","authenticated-orcid":false,"given":"Wenting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medicine Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2004.023804"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000006742"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2012.03501.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh282"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16159"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181bb1dfd"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2010.212"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2018-319396"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11040748"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2012.116"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.450040510"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz209"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1002-20.2021"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10622"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3456-16.2017"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyt.2021.679960"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1255263"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.08.010"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.05.007"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4430"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2022.863003"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00145"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0445-9"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2022.05.009"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.13709"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0013-4694(72)90177-0"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2013.10.011"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41701"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-023-00710-z"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.09.020"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3934"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.17295"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.24135"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2016.56"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/neuro.02.005.2008"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62373"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06899-3"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.05.027"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2441"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2044"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00936-3"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2016.00150"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-016-0192-8"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.08.040"},{"issue":"6","key":"e_1_2_11_46_1","first-page":"420","article-title":"MECP2, a multi\u2010talented modulator of chromatin architecture","volume":"15","author":"Della Ragione F","year":"2016","journal-title":"Brief Funct Genomics"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.08.018"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2016.103"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14813-z"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.23455"}],"updated-by":[{"DOI":"10.1111\/epi.18363","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000}}],"container-title":["Epilepsia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/epi.18027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T10:52:18Z","timestamp":1723200738000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/epi.18027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":49,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/epi.18027"],"URL":"https:\/\/doi.org\/10.1111\/epi.18027","archive":["Portico"],"relation":{"correction":[{"id-type":"doi","id":"10.1111\/epi.18363","asserted-by":"object"}]},"ISSN":["0013-9580","1528-1167"],"issn-type":[{"value":"0013-9580","type":"print"},{"value":"1528-1167","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,31]]},"assertion":[{"value":"2024-01-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}