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To test this hypothesis, we established a novel mouse line permitting inducible ablation of pyramidal neurons by inserting simian diphtheria toxin (DT) receptor (DTR) cDNA into the <jats:italic>Ccl17<\/jats:italic> locus. The chemokine CCL17 is expressed in pyramidal CA1 neurons in adult mice controlling microglial quiescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seizure activity in CCL17\u2010DTR mice was analyzed by electroencephalographic recordings following treatment with DT for 3 consecutive days. Neuroinflammation and neuronal cell death were evaluated by (immuno)histochemistry. Pharmacological inhibition of TNFR1 signaling was achieved by treatment with XPro1595, a dominant\u2010negative inhibitor of soluble tumor necrosis factor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neuronal cell death was detectable\u00a07\u2009days (d7) after the first DT injection in heterozygous CCL17\u2010DTR mice. Spontaneous epileptic seizures were observed in the vast majority of mice, often with an initial peak at d6\u20139, followed by a period of reduced activity and a gradual increase during the 1\u2010month observation period. Microglial reactivity was overt from d5 after DT administration not only in the CA1 region but also in the CA2\/CA3 area, shortly followed by astrogliosis. Reactive microgliosis and astrogliosis persisted until d30 and, together with neuronal loss and stratum radiatum shrinkage, reflected important features of human hippocampal sclerosis. Granule cell dispersion was detectable only 3\u2009months after DT treatment. Application of XPro1595 significantly reduced chronic seizure burden without affecting the development of hippocampal sclerosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>In conclusion, our data demonstrate that sterile pyramidal neuronal death is sufficient to cause epilepsy in the absence of other pathological processes. The CCL17\u2010DTR mouse line may thus be a valuable model for further mechanistic studies on epilepsy and assessment of antiseizure medication.<\/jats:p><\/jats:sec>","DOI":"10.1111\/epi.18200","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T15:02:01Z","timestamp":1732806121000},"page":"554-568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Ablation of <scp>CCL17<\/scp>\u2010positive hippocampal neurons induces inflammation\u2010dependent epilepsy"],"prefix":"10.1111","volume":"66","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3445-6397","authenticated-orcid":false,"given":"Judith","family":"Eberhard","sequence":"first","affiliation":[{"name":"Immunology &amp; Environment, Life and Medical Sciences Institute University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4749-5162","authenticated-orcid":false,"given":"Lukas","family":"Henning","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences, Medical Faculty University of Bonn  Bonn Germany"},{"name":"Deutsche Forschungsgemeinschaft  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2087-2984","authenticated-orcid":false,"given":"Lorenz","family":"F\u00fclle","sequence":"additional","affiliation":[{"name":"Immunology &amp; 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