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Although previous studies have implicated somatic mutations in the\n                      <jats:italic>SLC35A2<\/jats:italic>\n                      gene, the cellular and molecular mechanisms underlying MOGHE pathogenesis remain elusive. To address this gap, this study aimed to systematically characterize the cell type composition and molecular alterations of MOGHE lesions at cellular resolution using single\u2010nucleus multiomic profiling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed single\u2010nucleus multiomic sequencing to obtain paired gene expression and chromatin accessibility profiles of &gt;31\u2009000 nuclei from gray matter and white matter regions of MOGHE lesions and compared the results with publicly available neurotypical control datasets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The analysis of gray and white matter regions from two MOGHE patients revealed significant cellular composition alterations, including the presence of heterotopic neurons and disease\u2010specific oligodendrocyte populations within the subcortical white matter. MOGHE\u2010specific oligodendrocytes were characterized by the upregulation of synaptic functions and enhanced neuron communication, denoting a possible role in synaptic support and the mediation of glia\u2013neuron interactions in the disease. On the other hand, MOGHE heterotopic neurons were characterized by the upregulation of genes associated with neuronal migration and the Wnt signaling pathway, suggesting a mechanism underlying their atypical localization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance<\/jats:title>\n                    <jats:p>This high\u2010resolution cell type mapping of MOGHE lesions in clinical samples unveils neuronal and glial populations affected by the disease and provides novel insights into the pathophysiological mechanisms of MOGHE.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/epi.18413","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T07:25:35Z","timestamp":1745825135000},"page":"3064-3080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cell type mapping of mild malformations of cortical development with oligodendroglial hyperplasia in epilepsy using single\u2010nucleus multiomics"],"prefix":"10.1111","volume":"66","author":[{"given":"Isabella C.","family":"Galv\u00e3o","sequence":"first","affiliation":[{"name":"Department of Medical Genetics and Genomic Medicine School of Medical Sciences, University of Campinas  Campinas Brazil"},{"name":"Brazilian Institute of Neuroscience and Neurotechnology, Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Lemoine","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics and Genomic Medicine School of Medical Sciences, University of Campinas  Campinas Brazil"},{"name":"Brazilian Institute of Neuroscience and Neurotechnology, Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmyla","family":"Kandratavicius","sequence":"additional","affiliation":[{"name":"Brazilian Institute of Neuroscience and Neurotechnology, Campinas  Campinas Brazil"},{"name":"Department of Pathology School of Medical Sciences, University of Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa L.","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Brazilian Institute of Neuroscience and Neurotechnology, Campinas  Campinas Brazil"},{"name":"Department of Neurology School of Medical Sciences, University of Campinas  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina K. 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