{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:08:59Z","timestamp":1787760539044,"version":"build-2784847793"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC\u20102023\u2010COG\u2010101125118 (EPIAROUSAL)."],"award-info":[{"award-number":["ERC\u20102023\u2010COG\u2010101125118 (EPIAROUSAL)."]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["Contrat d\u2019interface Hospitalier 2018"],"award-info":[{"award-number":["Contrat d\u2019interface Hospitalier 2018"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Epilepsia"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study was undertaken to evaluate the diagnostic value of a set of preselected candidate microRNAs (miRNAs) extracted from plasma\u2010based extracellular vesicles (EVs) to identify patients with seizure\u2010related respiratory dysfunction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A two\u2010step design was applied. Step 1 entailed selection of the relevant miRNAs based on the combination of a literature review and an exploratory study in epileptic rats with or without interictal respiratory dysfunction. Step 2 involved evaluation of the diagnostic value of this preselected panel of circulating exosomal miRNAs in a case\u2013control study conducted in 25 healthy subjects and 50 patients with drug\u2010resistant focal epilepsy undergoing video\u2010electroencephalographic (EEG) monitoring. Based on video\u2010EEG data, patients were separated into two groups: those with ictal\/postictal hypoxemia (PIH;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200924) and those without (noPIH;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200926). Blood samples were collected in the interictal period (&gt;24\u2009h after the last seizure). Expression level of each miRNAs in EVs was compared (1) between all patients with epilepsy and controls and (2) between PIH and noPIH. Receiver operating characteristic (ROC) curves were generated, and the area under the curve (AUC) was calculated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Following Step 1, the final set of miRNAs selected for evaluation in the case\u2013control study included 24 miRNAs, with nine selected from published data in patients because of their potential regulatory role in the serotoninergic pathway, brain response to hypoxia, or epilepsy and 15 selected from the preclinical study in epileptic rats. Three miRNAs significantly differed between patients with epilepsy and controls (ROC curve AUC: hsa\u2010miR\u201022\u20103p, .74 [95% confidence interval (CI) = .63\u2013.85]; hsa\u2010miR\u2010106b\u20105p, .69 [95% CI = .57\u2013.82]; and hsa\u2010miR\u201026a\u20105p, .72 [95% CI = .58\u2013.85]). Only a trend toward higher expression levels was observed for hsa\u2010miR\u2010140\u20103p in PIH compared to noPIH (+5%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.064).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Significance<\/jats:title>\n                    <jats:p>Whereas three miRNAs were robustly associated with epilepsy, none was significantly associated with seizure\u2010related respiratory dysfunction. Additional studies are required, including analysis of the expression of plasmatic cell\u2010free miRNAs, especially the miRNAs associated with interictal respiratory dysfunction in epileptic rats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/epi.18641","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T14:12:12Z","timestamp":1758204732000},"page":"408-423","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Extracellular vesicle\n                    <scp>microRNAs<\/scp>\n                    are biomarkers of focal epilepsy but not epilepsy\u2010related respiratory dysfunction"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4663-8515","authenticated-orcid":false,"given":"Sylvain","family":"Rheims","sequence":"first","affiliation":[{"name":"Department of Functional Neurology and Epileptology Hospices Civils de Lyon and Lyon 1 University  Lyon France"},{"name":"Lyon Neuroscience Research Center INSERM U1028\/CNRS UMR 5292\/Lyon 1 University  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1443-0645","authenticated-orcid":false,"given":"Hayet","family":"Kouchi","sequence":"additional","affiliation":[{"name":"Lyon Neuroscience Research Center INSERM U1028\/CNRS UMR 5292\/Lyon 1 University  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Busato","sequence":"additional","affiliation":[{"name":"Laboratory for Epigenetics &amp; Environment, Centre National de Recherche en G\u00e9nomique Humaine, CEA\u2013Institut de Biologie Fran\u00e7ois Jacob Universit\u00e9 Paris\u2010Saclay  Evry France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2916-1302","authenticated-orcid":false,"given":"Stanislas","family":"Lagarde","sequence":"additional","affiliation":[{"name":"Epileptology and Cerebral Rhythmology Assistance\u2010Publique H\u00f4pitaux de Marseille, Timone Hospital  Marseille France"},{"name":"INSERM, Institute Neurosciences des Syst\u00e8mes Aix Marseille Universit\u00e9  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Derbala","sequence":"additional","affiliation":[{"name":"Laboratory for Epigenetics &amp; Environment, Centre National de Recherche en G\u00e9nomique Humaine, CEA\u2013Institut de Biologie Fran\u00e7ois Jacob Universit\u00e9 Paris\u2010Saclay  Evry France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8681-7369","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Boulogne","sequence":"additional","affiliation":[{"name":"Department of Functional Neurology and Epileptology Hospices Civils de Lyon and Lyon 1 University  Lyon France"},{"name":"Lyon Neuroscience Research Center INSERM U1028\/CNRS UMR 5292\/Lyon 1 University  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Department of Functional Neurology and Epileptology Hospices Civils de Lyon and Lyon 1 University  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Chenais","sequence":"additional","affiliation":[{"name":"Laboratory for Epigenetics &amp; Environment, Centre National de Recherche en G\u00e9nomique Humaine, CEA\u2013Institut de Biologie Fran\u00e7ois Jacob Universit\u00e9 Paris\u2010Saclay  Evry France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Bouvard","sequence":"additional","affiliation":[{"name":"Lyon Neuroscience Research Center INSERM U1028\/CNRS UMR 5292\/Lyon 1 University  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1678-0297","authenticated-orcid":false,"given":"Fabrice","family":"Bartolomei","sequence":"additional","affiliation":[{"name":"Epileptology and Cerebral Rhythmology Assistance\u2010Publique H\u00f4pitaux de Marseille, Timone Hospital  Marseille France"},{"name":"INSERM, Institute Neurosciences des Syst\u00e8mes Aix Marseille Universit\u00e9  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6411-4267","authenticated-orcid":false,"given":"Laurent","family":"Bezin","sequence":"additional","affiliation":[{"name":"Lyon Neuroscience Research Center INSERM U1028\/CNRS UMR 5292\/Lyon 1 University  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorg","family":"Tost","sequence":"additional","affiliation":[{"name":"Laboratory for Epigenetics &amp; Environment, Centre National de Recherche en G\u00e9nomique Humaine, CEA\u2013Institut de Biologie Fran\u00e7ois Jacob Universit\u00e9 Paris\u2010Saclay  Evry France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(16)30158-2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1010481"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60176-1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000000668"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.035"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-020-0369-8"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(16)30246-0"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1320"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09522"},{"key":"e_1_2_10_11_1","article-title":"Circulating microRNAs are promising novel biomarkers for drug\u2010resistant epilepsy","volume":"5","author":"Wang J","year":"2015","journal-title":"Sci Rep"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2020.105048"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.11.029"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-14153-6"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.18006"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2003.10.010"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2019.113145"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.17812"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awy078"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2009.02397.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3999(01)00296-3"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12888-014-0354-0"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000006777"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-6-r64"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-173"},{"key":"e_1_2_10_26_1","article-title":"Requirements for minimum sample size for sensitivity and specificity analysis","volume":"10","author":"Bujang MA","year":"2016","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15497"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.13236"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.16837"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.17521"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.910662"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20187566"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105476"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2009.02518.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awn277"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12153"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2019.00166"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2022.11.007"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0603-0"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-024-03162-z"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000008741"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(13)70214-X"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000008244"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.10.068"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7979500"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2020.584828"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2015.1071871"}],"container-title":["Epilepsia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/epi.18641","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/epi.18641","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/epi.18641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:12:08Z","timestamp":1773000728000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/epi.18641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,18]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/epi.18641"],"URL":"https:\/\/doi.org\/10.1111\/epi.18641","archive":["Portico"],"relation":{},"ISSN":["0013-9580","1528-1167"],"issn-type":[{"value":"0013-9580","type":"print"},{"value":"1528-1167","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,18]]},"assertion":[{"value":"2025-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}